TY  - JOUR
AU  - Hilgers, Fabienne
AU  - Bitzenhofer, Nora Lisa
AU  - Ackermann, Yannic
AU  - Burmeister, Alina
AU  - Grünberger, Alexander
AU  - Jaeger, Karl-Erich
AU  - Drepper, Thomas
TI  - Genetically encoded photosensitizers as light-triggered antimicrobial agents
JO  - International journal of molecular sciences
VL  - 20
IS  - 18
SN  - 1422-0067
CY  - Basel
PB  - Molecular Diversity Preservation International
M1  - FZJ-2019-05251
SP  - 4608 -
PY  - 2019
AB  - Diseases caused by multi-drug resistant pathogens have become a global concern. Therefore, new approaches suitable for treating these bacteria are urgently needed. In this study, we analyzed genetically encoded photosensitizers (PS) related to the green fluorescent protein (GFP) or light-oxygen-voltage (LOV) photoreceptors for their exogenous applicability as light-triggered antimicrobial agents. Depending on their specific photophysical properties and photochemistry, these PSs can produce different toxic ROS (reactive oxygen species) such as O2•− and H2O2 via type-I, as well as 1O2 via type-II reaction in response to light. By using cell viability assays and microfluidics, we could demonstrate differences in the intracellular and extracellular phototoxicity of the applied PS. While intracellular expression and exogenous supply of GFP-related PSs resulted in a slow inactivation of E. coli and pathogenic Gram-negative and Gram-positive bacteria, illumination of LOV-based PSs such as the singlet oxygen photosensitizing protein SOPP3 resulted in a fast and homogeneous killing of these microbes. Furthermore, our data indicate that the ROS type and yield as well as the localization of the applied PS protein can strongly influence the antibacterial spectrum and efficacy. These findings open up new opportunities for photodynamic inactivation of pathogenic bacteria
LB  - PUB:(DE-HGF)16
C6  - pmid:31533368
UR  - <Go to ISI:>//WOS:000489100500287
DO  - DOI:10.3390/ijms20184608
UR  - https://juser.fz-juelich.de/record/865990
ER  -