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@ARTICLE{Hilgers:865990,
      author       = {Hilgers, Fabienne and Bitzenhofer, Nora Lisa and Ackermann,
                      Yannic and Burmeister, Alina and Grünberger, Alexander and
                      Jaeger, Karl-Erich and Drepper, Thomas},
      title        = {{G}enetically encoded photosensitizers as light-triggered
                      antimicrobial agents},
      journal      = {International journal of molecular sciences},
      volume       = {20},
      number       = {18},
      issn         = {1422-0067},
      address      = {Basel},
      publisher    = {Molecular Diversity Preservation International},
      reportid     = {FZJ-2019-05251},
      pages        = {4608 -},
      year         = {2019},
      abstract     = {Diseases caused by multi-drug resistant pathogens have
                      become a global concern. Therefore, new approaches suitable
                      for treating these bacteria are urgently needed. In this
                      study, we analyzed genetically encoded photosensitizers (PS)
                      related to the green fluorescent protein (GFP) or
                      light-oxygen-voltage (LOV) photoreceptors for their
                      exogenous applicability as light-triggered antimicrobial
                      agents. Depending on their specific photophysical properties
                      and photochemistry, these PSs can produce different toxic
                      ROS (reactive oxygen species) such as O2•− and H2O2 via
                      type-I, as well as 1O2 via type-II reaction in response to
                      light. By using cell viability assays and microfluidics, we
                      could demonstrate differences in the intracellular and
                      extracellular phototoxicity of the applied PS. While
                      intracellular expression and exogenous supply of GFP-related
                      PSs resulted in a slow inactivation of E. coli and
                      pathogenic Gram-negative and Gram-positive bacteria,
                      illumination of LOV-based PSs such as the singlet oxygen
                      photosensitizing protein SOPP3 resulted in a fast and
                      homogeneous killing of these microbes. Furthermore, our data
                      indicate that the ROS type and yield as well as the
                      localization of the applied PS protein can strongly
                      influence the antibacterial spectrum and efficacy. These
                      findings open up new opportunities for photodynamic
                      inactivation of pathogenic bacteria},
      cin          = {IMET / IBG-1},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IMET-20090612 / I:(DE-Juel1)IBG-1-20101118},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31533368},
      UT           = {WOS:000489100500287},
      doi          = {10.3390/ijms20184608},
      url          = {https://juser.fz-juelich.de/record/865990},
}