TY  - JOUR
AU  - Schell, Christoph
AU  - Sabass, Benedikt
AU  - Helmstaedter, Martin
AU  - Geist, Felix
AU  - Abed, Ahmed
AU  - Yasuda-Yamahara, Mako
AU  - Sigle, August
AU  - Maier, Jasmin I.
AU  - Grahammer, Florian
AU  - Siegerist, Florian
AU  - Artelt, Nadine
AU  - Endlich, Nicole
AU  - Kerjaschki, Dontscho
AU  - Arnold, Hans-Henning
AU  - Dengjel, Jörn
AU  - Rogg, Manuel
AU  - Huber, Tobias B.
TI  - ARP3 Controls the Podocyte Architecture at the Kidney Filtration Barrier
JO  - Developmental cell
VL  - 47
IS  - 6
SN  - 1534-5807
CY  - New York, NY
PB  - Elsevier
M1  - FZJ-2019-05345
SP  - 741 - 757.e8
PY  - 2018
AB  - Podocytes, highly specialized epithelial cells, build the outer part of the kidney filtration barrier and withstand high mechanical forces through a complex network of cellular protrusions. Here, we show that Arp2/3-dependent actin polymerization controls actomyosin contractility and focal adhesion maturation of podocyte protrusions and thereby regulates formation, maintenance, and capacity to adapt to mechanical requirements of the filtration barrier. We find that N-WASP-Arp2/3 define the development of complex arborized podocyte protrusions in vitro and in vivo. Loss of dendritic actin networks results in a pronounced activation of the actomyosin cytoskeleton and the generation of over-maturated but less efficient adhesion, leading to detachment of podocytes. Our data provide a model to explain podocyte protrusion morphology and their mechanical stability based on a tripartite relationship between actin polymerization, contractility, and adhesion.
LB  - PUB:(DE-HGF)16
C6  - pmid:30503751
UR  - <Go to ISI:>//WOS:000453390700015
DO  - DOI:10.1016/j.devcel.2018.11.011
UR  - https://juser.fz-juelich.de/record/866149
ER  -