000866579 001__ 866579
000866579 005__ 20210130003529.0
000866579 037__ $$aFZJ-2019-05662
000866579 041__ $$aEnglish
000866579 1001_ $$0P:(DE-Juel1)165155$$aArsova, Borjana$$b0$$eCorresponding author$$ufzj
000866579 1112_ $$aMicrobe-assisted crop production – opportunities, challenges and needs$$cVienna$$d2019-12-02 - 2019-12-05$$gmiCROPe 2019$$wAustria
000866579 245__ $$aThe impact of beneficial microbes on Brachypodium nutrient uptake under limiting supplies of nitrogen and phosphorus, monitored with non-invasive phenotyping and molecular approaches
000866579 260__ $$c2019
000866579 3367_ $$033$$2EndNote$$aConference Paper
000866579 3367_ $$2DataCite$$aOther
000866579 3367_ $$2BibTeX$$aINPROCEEDINGS
000866579 3367_ $$2DRIVER$$aconferenceObject
000866579 3367_ $$2ORCID$$aLECTURE_SPEECH
000866579 3367_ $$0PUB:(DE-HGF)6$$2PUB:(DE-HGF)$$aConference Presentation$$bconf$$mconf$$s1574325583_25086$$xInvited
000866579 520__ $$aIn times of increasing global population and decreasing arable land per capita, the understanding of plant nutrient uptake and novel strategies to improve nutrient uptake are of utmost importance. Our work focuses on nitrogen (N) – the second most abundant nutrient in plants and phosphorus (P) – a finite global resource. We present studies where use of plant growth promoting rhizobacteria (PGPR) resulted in improved plant performance under limited N or P in Brachypodium- a model plant for cereals. Plant roots were analyzed with the non-invasive root phenotyping platform GrowScreen Page [1], or with the 3D printed EcoFab microcosms [2]. The latter was adapted and used in combination with Plant Screen Mobile [3], for non-invasive shoot area estimation, in conjunction with root scanning, over time.In the case of P limitation, plant biomass was higher in plants inoculated with a PGPR. A time series image-analysis of root phenotype allowed visualization of increased root length and changes in root architecture, pin-pointing the time-window when growth promotion took effect after inoculation. A sand experiment similarly resulted in increased biomass in inoculated plants. Study of the molecular mechanisms behind this whole plant, dynamic phenotype is ongoing and involves metabolomics and lipidomics.In the case where plants with limiting N supply were inoculated with N-fixing PGPR, an end-point harvest showed that ratio of lateral to primary root length increases. More importantly, N concentration in root and shoot tissue increased, along with greater shoot biomass and leaf area. We complemented this destructive harvest with proteomics to investigate the systemic response of Brachypodium constitutively grown under limiting N, to the interaction with the PGPR. Data analysis revealed that these N-fixing bacteria impact central nitrogen metabolism in Brachypodium, and indicate a mode of action that upregulates specific N transporters on the root plasma membrane.The grass model can thus clearly benefit from PGPR, however the time points, tissue responses and molecular mechanisms were different for organisms and nutrient conditions. Efforts are needed to elucidate plant responses to the microorganisms, addressing molecular and tissue architecture, while taking in context plant developmental stage [4] and time since application. 1.	Funct Plant Biol, 2017. 44(1)2.	New Phytol. 2019; 222(2): 1149–1160 3.	Plant Methods 2019 15:2 4.	New Phytol. 2019 doi: 10.1111/nph.15955
000866579 536__ $$0G:(DE-HGF)POF3-582$$a582 - Plant Science (POF3-582)$$cPOF3-582$$fPOF III$$x0
000866579 7001_ $$0P:(DE-Juel1)178056$$aSanow, Stefan$$b1$$ufzj
000866579 7001_ $$0P:(DE-HGF)0$$aSchillaci, Martino$$b2
000866579 7001_ $$0P:(DE-Juel1)174213$$akuang, weiqi$$b3$$ufzj
000866579 7001_ $$0P:(DE-Juel1)162356$$aHuesgen, Pitter$$b4$$ufzj
000866579 7001_ $$0P:(DE-HGF)0$$aRoessner, Ute$$b5
000866579 7001_ $$0P:(DE-Juel1)166460$$aWatt, Michelle$$b6$$ufzj
000866579 909CO $$ooai:juser.fz-juelich.de:866579$$pVDB
000866579 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)165155$$aForschungszentrum Jülich$$b0$$kFZJ
000866579 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)178056$$aForschungszentrum Jülich$$b1$$kFZJ
000866579 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)174213$$aForschungszentrum Jülich$$b3$$kFZJ
000866579 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)162356$$aForschungszentrum Jülich$$b4$$kFZJ
000866579 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)166460$$aForschungszentrum Jülich$$b6$$kFZJ
000866579 9131_ $$0G:(DE-HGF)POF3-582$$1G:(DE-HGF)POF3-580$$2G:(DE-HGF)POF3-500$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bKey Technologies$$lKey Technologies for the Bioeconomy$$vPlant Science$$x0
000866579 9141_ $$y2019
000866579 9201_ $$0I:(DE-Juel1)IBG-2-20101118$$kIBG-2$$lPflanzenwissenschaften$$x0
000866579 9201_ $$0I:(DE-Juel1)ZEA-3-20090406$$kZEA-3$$lAnalytik$$x1
000866579 980__ $$aconf
000866579 980__ $$aVDB
000866579 980__ $$aI:(DE-Juel1)IBG-2-20101118
000866579 980__ $$aI:(DE-Juel1)ZEA-3-20090406
000866579 980__ $$aUNRESTRICTED