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@INPROCEEDINGS{Arsova:866579,
      author       = {Arsova, Borjana and Sanow, Stefan and Schillaci, Martino
                      and kuang, weiqi and Huesgen, Pitter and Roessner, Ute and
                      Watt, Michelle},
      title        = {{T}he impact of beneficial microbes on {B}rachypodium
                      nutrient uptake under limiting supplies of nitrogen and
                      phosphorus, monitored with non-invasive phenotyping and
                      molecular approaches},
      reportid     = {FZJ-2019-05662},
      year         = {2019},
      abstract     = {In times of increasing global population and decreasing
                      arable land per capita, the understanding of plant nutrient
                      uptake and novel strategies to improve nutrient uptake are
                      of utmost importance. Our work focuses on nitrogen (N) –
                      the second most abundant nutrient in plants and phosphorus
                      (P) – a finite global resource. We present studies where
                      use of plant growth promoting rhizobacteria (PGPR) resulted
                      in improved plant performance under limited N or P in
                      Brachypodium- a model plant for cereals. Plant roots were
                      analyzed with the non-invasive root phenotyping platform
                      GrowScreen Page [1], or with the 3D printed EcoFab
                      microcosms [2]. The latter was adapted and used in
                      combination with Plant Screen Mobile [3], for non-invasive
                      shoot area estimation, in conjunction with root scanning,
                      over time.In the case of P limitation, plant biomass was
                      higher in plants inoculated with a PGPR. A time series
                      image-analysis of root phenotype allowed visualization of
                      increased root length and changes in root architecture,
                      pin-pointing the time-window when growth promotion took
                      effect after inoculation. A sand experiment similarly
                      resulted in increased biomass in inoculated plants. Study of
                      the molecular mechanisms behind this whole plant, dynamic
                      phenotype is ongoing and involves metabolomics and
                      lipidomics.In the case where plants with limiting N supply
                      were inoculated with N-fixing PGPR, an end-point harvest
                      showed that ratio of lateral to primary root length
                      increases. More importantly, N concentration in root and
                      shoot tissue increased, along with greater shoot biomass and
                      leaf area. We complemented this destructive harvest with
                      proteomics to investigate the systemic response of
                      Brachypodium constitutively grown under limiting N, to the
                      interaction with the PGPR. Data analysis revealed that these
                      N-fixing bacteria impact central nitrogen metabolism in
                      Brachypodium, and indicate a mode of action that upregulates
                      specific N transporters on the root plasma membrane.The
                      grass model can thus clearly benefit from PGPR, however the
                      time points, tissue responses and molecular mechanisms were
                      different for organisms and nutrient conditions. Efforts are
                      needed to elucidate plant responses to the microorganisms,
                      addressing molecular and tissue architecture, while taking
                      in context plant developmental stage [4] and time since
                      application. 1. Funct Plant Biol, 2017. 44(1)2. New Phytol.
                      2019; 222(2): 1149–1160 3. Plant Methods 2019 15:2 4. New
                      Phytol. 2019 doi: 10.1111/nph.15955},
      month         = {Dec},
      date          = {2019-12-02},
      organization  = {Microbe-assisted crop production –
                       opportunities, challenges and needs,
                       Vienna (Austria), 2 Dec 2019 - 5 Dec
                       2019},
      subtyp        = {Invited},
      cin          = {IBG-2 / ZEA-3},
      cid          = {I:(DE-Juel1)IBG-2-20101118 / I:(DE-Juel1)ZEA-3-20090406},
      pnm          = {582 - Plant Science (POF3-582)},
      pid          = {G:(DE-HGF)POF3-582},
      typ          = {PUB:(DE-HGF)6},
      url          = {https://juser.fz-juelich.de/record/866579},
}