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@ARTICLE{Stoffels:866707,
      author       = {Stoffels, Peter and Müller, Markus Jan and Stachurski,
                      Sarah and Terfrüchte, Marius and Schröder, Sebastian and
                      Ihling, Nina and Wierckx, Nick and Feldbrügge, Michael and
                      Schipper, Kerstin and Büchs, Jochen},
      title        = {{C}omplementing the intrinsic repertoire of {U}stilago
                      maydis for degradation of the pectin backbone
                      polygalacturonic acid},
      journal      = {Journal of biotechnology},
      volume       = {307},
      issn         = {0168-1656},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {FZJ-2019-05777},
      pages        = {148 - 163},
      year         = {2020},
      note         = {Biotechnologie 1},
      abstract     = {Microbial valorization of plant biomass is a key target in
                      bioeconomy. A promising candidate for consolidated
                      bioprocessing is the dimorphic fungus Ustilago maydis. It
                      harbors hydrolytic enzymes to degrade biomass components and
                      naturally produces valuable secondary metabolites like
                      itaconic acid, malic acid or glycolipids. However,
                      hydrolytic enzymes are mainly expressed in the hyphal form.
                      This type of morphology should be prevented in industrial
                      fermentation processes. Genetic activation of these enzymes
                      can enable growth on cognate substrates also in the yeast
                      form. Here, strains were engineered for growth on
                      polygalacturonic acid as major component of pectin. Besides
                      activation of intrinsic enzymes, supplementation with
                      heterologous genes for potent enzymes was tested. The
                      presence of an unconventional secretion pathway allowed
                      exploiting fungal and bacterial enzymes. Growth of the
                      engineered strains was evaluated by a recently developed
                      method for online determination of residual substrates based
                      on the respiration activity. This enabled the quantification
                      of the overall consumed substrate as a key asset for the
                      assessment of the enzyme degradation potential even on
                      polymeric substrates. Co-fermentation of endo- and
                      exo-polygalacturonase overexpression strains resulted in
                      efficient growth on polygalacturonic acid. In the future,
                      the approach will be extended to establish efficient
                      degradation and valorization of pectin. Previous article in
                      issue},
      cin          = {IBG-1},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31715206},
      UT           = {WOS:000502527100017},
      doi          = {10.1016/j.jbiotec.2019.10.022},
      url          = {https://juser.fz-juelich.de/record/866707},
}