TY  - JOUR
AU  - Morschett, Holger
AU  - Jansen, Roman
AU  - Neuendorf, Christian
AU  - Moch, Matthias
AU  - Wiechert, Wolfgang
AU  - Oldiges, Marco
TI  - Parallelized microscale fed-batch cultivation in online-monitored microtiter plates: implications of media composition and feed strategies for process design and performance
JO  - Journal of industrial microbiology & biotechnology
VL  - 47
SN  - 1476-5535
CY  - Berlin
PB  - Springer67420
M1  - FZJ-2019-05784
SP  - 35-47
PY  - 2020
AB  - Limited throughput represents a substantial drawback during bioprocess development. In recent years, several commercial microbioreactor systems have emerged featuring parallelized experimentation with optical monitoring. However, many devices remain limited to batch mode and do not represent the fed-batch strategy typically applied on an industrial scale. A workflow for 32-fold parallelized microscale cultivation of protein secreting Corynebacterium glutamicum in microtiter plates incorporating online monitoring, pH control and feeding was developed and validated. Critical interference of the essential media component protocatechuic acid with pH measurement was revealed, but was effectively resolved by 80% concentration reduction without affecting biological performance. Microfluidic pH control and feeding (pulsed, constant and exponential) were successfully implemented: Whereas pH control improved performance only slightly, feeding revealed a much higher optimization potential. Exponential feeding with µ = 0.1 h−1 resulted in the highest product titers. In contrast, other performance indicators such as biomass-specific or volumetric productivity resulted in different optimal feeding regimes.
LB  - PUB:(DE-HGF)16
C6  - pmid:31673873
UR  - <Go to ISI:>//WOS:000493750500001
DO  - DOI:10.1007/s10295-019-02243-w
UR  - https://juser.fz-juelich.de/record/866714
ER  -