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@ARTICLE{Morschett:866714,
      author       = {Morschett, Holger and Jansen, Roman and Neuendorf,
                      Christian and Moch, Matthias and Wiechert, Wolfgang and
                      Oldiges, Marco},
      title        = {{P}arallelized microscale fed-batch cultivation in
                      online-monitored microtiter plates: implications of media
                      composition and feed strategies for process design and
                      performance},
      journal      = {Journal of industrial microbiology $\&$ biotechnology},
      volume       = {47},
      issn         = {1476-5535},
      address      = {Berlin},
      publisher    = {Springer67420},
      reportid     = {FZJ-2019-05784},
      pages        = {35-47},
      year         = {2020},
      abstract     = {Limited throughput represents a substantial drawback during
                      bioprocess development. In recent years, several commercial
                      microbioreactor systems have emerged featuring parallelized
                      experimentation with optical monitoring. However, many
                      devices remain limited to batch mode and do not represent
                      the fed-batch strategy typically applied on an industrial
                      scale. A workflow for 32-fold parallelized microscale
                      cultivation of protein secreting Corynebacterium glutamicum
                      in microtiter plates incorporating online monitoring, pH
                      control and feeding was developed and validated. Critical
                      interference of the essential media component protocatechuic
                      acid with pH measurement was revealed, but was effectively
                      resolved by $80\%$ concentration reduction without affecting
                      biological performance. Microfluidic pH control and feeding
                      (pulsed, constant and exponential) were successfully
                      implemented: Whereas pH control improved performance only
                      slightly, feeding revealed a much higher optimization
                      potential. Exponential feeding with µ = 0.1 h−1 resulted
                      in the highest product titers. In contrast, other
                      performance indicators such as biomass-specific or
                      volumetric productivity resulted in different optimal
                      feeding regimes.},
      cin          = {IBG-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31673873},
      UT           = {WOS:000493750500001},
      doi          = {10.1007/s10295-019-02243-w},
      url          = {https://juser.fz-juelich.de/record/866714},
}