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000866840 1001_ $$0P:(DE-Juel1)165927$$aHöfig, Henning$$b0
000866840 245__ $$aBrightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation
000866840 260__ $$aLondon$$bSpringer Nature$$c2019
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000866840 520__ $$aLife on the molecular scale is based on a complex interplay of biomolecules under which the ability of binding is crucial. Fluorescence based two-color coincidence detection (TCCD) is commonly used to characterize molecular binding, but suffers from an underestimation of coincident events. Here, we introduce a brightness-gated TCCD which overcomes this limitation and benchmark our approach with two custom-made calibration samples. Applied to a cell-free protein synthesis assay, brightness-gated TCCD unraveled a previously disregarded mode of translation initiation in bacteria.
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000866840 7001_ $$0P:(DE-Juel1)176889$$aYukhnovets, Olessya$$b1$$ufzj
000866840 7001_ $$0P:(DE-Juel1)156351$$aRemes, Cristina$$b2
000866840 7001_ $$0P:(DE-Juel1)166089$$aKempf, Noemie$$b3
000866840 7001_ $$0P:(DE-Juel1)131971$$aKatranidis, Alexandros$$b4
000866840 7001_ $$0P:(DE-HGF)0$$aKempe, Daryan$$b5
000866840 7001_ $$0P:(DE-Juel1)131961$$aFitter, Jörg$$b6$$eCorresponding author
000866840 773__ $$0PERI:(DE-600)2919698-X$$a10.1038/s42003-019-0709-7$$gVol. 2, no. 1, p. 459$$n1$$p459$$tCommunications biology$$v2$$x2399-3642$$y2019
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