TY  - JOUR
AU  - Höfig, Henning
AU  - Yukhnovets, Olessya
AU  - Remes, Cristina
AU  - Kempf, Noemie
AU  - Katranidis, Alexandros
AU  - Kempe, Daryan
AU  - Fitter, Jörg
TI  - Brightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation
JO  - Communications biology
VL  - 2
IS  - 1
SN  - 2399-3642
CY  - London
PB  - Springer Nature
M1  - FZJ-2019-05903
SP  - 459
PY  - 2019
AB  - Life on the molecular scale is based on a complex interplay of biomolecules under which the ability of binding is crucial. Fluorescence based two-color coincidence detection (TCCD) is commonly used to characterize molecular binding, but suffers from an underestimation of coincident events. Here, we introduce a brightness-gated TCCD which overcomes this limitation and benchmark our approach with two custom-made calibration samples. Applied to a cell-free protein synthesis assay, brightness-gated TCCD unraveled a previously disregarded mode of translation initiation in bacteria.
LB  - PUB:(DE-HGF)16
C6  - pmid:31840104
UR  - <Go to ISI:>//WOS:000502374300006
DO  - DOI:10.1038/s42003-019-0709-7
UR  - https://juser.fz-juelich.de/record/866840
ER  -