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| Hauptseite > Workflowsammlungen > Publikationsgebühren > Brightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation > print |
| Hauptseite > Workflowsammlungen > Publikationsgebühren > Brightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation > print |
| 001 | 866840 | ||
| 005 | 20240610120502.0 | ||
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| 100 | 1 | _ | |a Höfig, Henning |0 P:(DE-Juel1)165927 |b 0 |
| 245 | _ | _ | |a Brightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation |
| 260 | _ | _ | |a London |c 2019 |b Springer Nature |
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| 520 | _ | _ | |a Life on the molecular scale is based on a complex interplay of biomolecules under which the ability of binding is crucial. Fluorescence based two-color coincidence detection (TCCD) is commonly used to characterize molecular binding, but suffers from an underestimation of coincident events. Here, we introduce a brightness-gated TCCD which overcomes this limitation and benchmark our approach with two custom-made calibration samples. Applied to a cell-free protein synthesis assay, brightness-gated TCCD unraveled a previously disregarded mode of translation initiation in bacteria. |
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| 773 | _ | _ | |a 10.1038/s42003-019-0709-7 |g Vol. 2, no. 1, p. 459 |0 PERI:(DE-600)2919698-X |n 1 |p 459 |t Communications biology |v 2 |y 2019 |x 2399-3642 |
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