000867572 001__ 867572
000867572 005__ 20210130003802.0
000867572 0247_ $$2doi$$a10.1021/acs.biomac.9b00184
000867572 0247_ $$2ISSN$$a1525-7797
000867572 0247_ $$2ISSN$$a1526-4602
000867572 0247_ $$2altmetric$$aaltmetric:59738270
000867572 0247_ $$2pmid$$apmid:30933481
000867572 0247_ $$2WOS$$aWOS:000468120800011
000867572 037__ $$aFZJ-2019-06195
000867572 082__ $$a570
000867572 1001_ $$00000-0001-6625-2284$$aRusso, Daniela$$b0$$eCorresponding author
000867572 245__ $$aEffect of Polymer Chain Density on Protein–Polymer Conjugate Conformation
000867572 260__ $$aColumbus, Ohio$$bAmerican Chemical Soc.$$c2019
000867572 3367_ $$2DRIVER$$aarticle
000867572 3367_ $$2DataCite$$aOutput Types/Journal article
000867572 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1576583683_32085
000867572 3367_ $$2BibTeX$$aARTICLE
000867572 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000867572 3367_ $$00$$2EndNote$$aJournal Article
000867572 520__ $$aMany biomedical applications employ covalent attachment to synthetic polymers to enhance the efficiency of proteins or other therapeutically active molecules. We report here the impact of polymer conjugation on the structural and thermal stability of a protein model, the bovine serum albumin, using a variable number of linear biodegradable polyphosphoesters, which were covalently tethered to the protein. We observed that BSA’s secondary structure measured by circular dichroism is independent of the conjugation. Small-angle neutron scattering, however, reveals a change from ellipsoid to globular shape of the whole complex arising from a slight compaction of the protein core and an increase of the polymer’s radius of gyration as a function of the grafting polymer density. In particular, we highlight a gradual change of the polymer conformation around the protein and elongation of the semimajor dimension of the ellipsoidal protein. Our results will contribute to the description of biophysical characteristics of a new class of biologically relevant protein–polymer conjugates
000867572 536__ $$0G:(DE-HGF)POF3-6G4$$a6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623)$$cPOF3-623$$fPOF III$$x0
000867572 536__ $$0G:(DE-HGF)POF3-6G15$$a6G15 - FRM II / MLZ (POF3-6G15)$$cPOF3-6G15$$fPOF III$$x1
000867572 588__ $$aDataset connected to CrossRef
000867572 65027 $$0V:(DE-MLZ)SciArea-210$$2V:(DE-HGF)$$aSoft Condensed Matter$$x0
000867572 65017 $$0V:(DE-MLZ)GC-1602-2016$$2V:(DE-HGF)$$aPolymers, Soft Nano Particles and  Proteins$$x0
000867572 693__ $$0EXP:(DE-MLZ)KWS2-20140101$$1EXP:(DE-MLZ)FRMII-20140101$$5EXP:(DE-MLZ)KWS2-20140101$$6EXP:(DE-MLZ)NL3ao-20140101$$aForschungs-Neutronenquelle Heinz Maier-Leibnitz $$eKWS-2: Small angle scattering diffractometer$$fNL3ao$$x0
000867572 7001_ $$0P:(DE-HGF)0$$ade Angelis, Andrea$$b1
000867572 7001_ $$0P:(DE-HGF)0$$aGarvey, Christopher. J.$$b2
000867572 7001_ $$00000-0002-6955-8489$$aWurm, Frederick R.$$b3
000867572 7001_ $$0P:(DE-Juel1)130507$$aAppavou, Marie-Sousai$$b4$$ufzj
000867572 7001_ $$0P:(DE-HGF)0$$aPrevost, Sylvain$$b5
000867572 773__ $$0PERI:(DE-600)2006291-6$$a10.1021/acs.biomac.9b00184$$gVol. 20, no. 5, p. 1944 - 1955$$n5$$p1944 - 1955$$tBiomacromolecules$$v20$$x1526-4602$$y2019
000867572 8564_ $$uhttps://juser.fz-juelich.de/record/867572/files/acs.biomac.9b00184-1.pdf$$yRestricted
000867572 8564_ $$uhttps://juser.fz-juelich.de/record/867572/files/acs.biomac.9b00184-1.pdf?subformat=pdfa$$xpdfa$$yRestricted
000867572 909CO $$ooai:juser.fz-juelich.de:867572$$pVDB$$pVDB:MLZ
000867572 9101_ $$0I:(DE-HGF)0$$60000-0001-6625-2284$$aExternal Institute$$b0$$kExtern
000867572 9101_ $$0I:(DE-HGF)0$$6P:(DE-HGF)0$$aExternal Institute$$b1$$kExtern
000867572 9101_ $$0I:(DE-HGF)0$$6P:(DE-HGF)0$$aExternal Institute$$b2$$kExtern
000867572 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)130507$$aForschungszentrum Jülich$$b4$$kFZJ
000867572 9101_ $$0I:(DE-HGF)0$$6P:(DE-HGF)0$$aExternal Institute$$b5$$kExtern
000867572 9131_ $$0G:(DE-HGF)POF3-623$$1G:(DE-HGF)POF3-620$$2G:(DE-HGF)POF3-600$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$9G:(DE-HGF)POF3-6G4$$aDE-HGF$$bForschungsbereich Materie$$lVon Materie zu Materialien und Leben$$vFacility topic: Neutrons for Research on Condensed Matter$$x0
000867572 9131_ $$0G:(DE-HGF)POF3-6G15$$1G:(DE-HGF)POF3-6G0$$2G:(DE-HGF)POF3-600$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$9G:(DE-HGF)POF3-6G15$$aDE-HGF$$bForschungsbereich Materie$$lGroßgeräte: Materie$$vFRM II / MLZ$$x1
000867572 9141_ $$y2019
000867572 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bBIOMACROMOLECULES : 2017
000867572 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS
000867572 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000867572 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database
000867572 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search
000867572 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC
000867572 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List
000867572 915__ $$0StatID:(DE-HGF)0110$$2StatID$$aWoS$$bScience Citation Index
000867572 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000867572 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000867572 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences
000867572 915__ $$0StatID:(DE-HGF)1150$$2StatID$$aDBCoverage$$bCurrent Contents - Physical, Chemical and Earth Sciences
000867572 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews
000867572 915__ $$0StatID:(DE-HGF)9905$$2StatID$$aIF >= 5$$bBIOMACROMOLECULES : 2017
000867572 920__ $$lyes
000867572 9201_ $$0I:(DE-Juel1)JCNS-FRM-II-20110218$$kJCNS-FRM-II$$lJCNS-FRM-II$$x0
000867572 9201_ $$0I:(DE-Juel1)JCNS-1-20110106$$kJCNS-1$$lNeutronenstreuung$$x1
000867572 9201_ $$0I:(DE-588b)4597118-3$$kMLZ$$lHeinz Maier-Leibnitz Zentrum$$x2
000867572 980__ $$ajournal
000867572 980__ $$aVDB
000867572 980__ $$aI:(DE-Juel1)JCNS-FRM-II-20110218
000867572 980__ $$aI:(DE-Juel1)JCNS-1-20110106
000867572 980__ $$aI:(DE-588b)4597118-3
000867572 980__ $$aUNRESTRICTED