TY  - JOUR
AU  - Russo, Daniela
AU  - de Angelis, Andrea
AU  - Garvey, Christopher. J.
AU  - Wurm, Frederick R.
AU  - Appavou, Marie-Sousai
AU  - Prevost, Sylvain
TI  - Effect of Polymer Chain Density on Protein–Polymer Conjugate Conformation
JO  - Biomacromolecules
VL  - 20
IS  - 5
SN  - 1526-4602
CY  - Columbus, Ohio
PB  - American Chemical Soc.
M1  - FZJ-2019-06195
SP  - 1944 - 1955
PY  - 2019
AB  - Many biomedical applications employ covalent attachment to synthetic polymers to enhance the efficiency of proteins or other therapeutically active molecules. We report here the impact of polymer conjugation on the structural and thermal stability of a protein model, the bovine serum albumin, using a variable number of linear biodegradable polyphosphoesters, which were covalently tethered to the protein. We observed that BSA’s secondary structure measured by circular dichroism is independent of the conjugation. Small-angle neutron scattering, however, reveals a change from ellipsoid to globular shape of the whole complex arising from a slight compaction of the protein core and an increase of the polymer’s radius of gyration as a function of the grafting polymer density. In particular, we highlight a gradual change of the polymer conformation around the protein and elongation of the semimajor dimension of the ellipsoidal protein. Our results will contribute to the description of biophysical characteristics of a new class of biologically relevant protein–polymer conjugates
LB  - PUB:(DE-HGF)16
C6  - pmid:30933481
UR  - <Go to ISI:>//WOS:000468120800011
DO  - DOI:10.1021/acs.biomac.9b00184
UR  - https://juser.fz-juelich.de/record/867572
ER  -