Home > Publications database > Effect of Polymer Chain Density on Protein–Polymer Conjugate Conformation > print

001     867572
005     20210130003802.0
024 7 _ |a 10.1021/acs.biomac.9b00184
|2 doi
024 7 _ |a 1525-7797
|2 ISSN
024 7 _ |a 1526-4602
|2 ISSN
024 7 _ |a altmetric:59738270
|2 altmetric
024 7 _ |a pmid:30933481
|2 pmid
024 7 _ |a WOS:000468120800011
|2 WOS
037 _ _ |a FZJ-2019-06195
082 _ _ |a 570
100 1 _ |a Russo, Daniela
|0 0000-0001-6625-2284
|b 0
|e Corresponding author
245 _ _ |a Effect of Polymer Chain Density on Protein–Polymer Conjugate Conformation
260 _ _ |a Columbus, Ohio
|c 2019
|b American Chemical Soc.
336 7 _ |a article
|2 DRIVER
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|b journal
|m journal
|0 PUB:(DE-HGF)16
|s 1576583683_32085
|2 PUB:(DE-HGF)
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a Journal Article
|0 0
|2 EndNote
520 _ _ |a Many biomedical applications employ covalent attachment to synthetic polymers to enhance the efficiency of proteins or other therapeutically active molecules. We report here the impact of polymer conjugation on the structural and thermal stability of a protein model, the bovine serum albumin, using a variable number of linear biodegradable polyphosphoesters, which were covalently tethered to the protein. We observed that BSA’s secondary structure measured by circular dichroism is independent of the conjugation. Small-angle neutron scattering, however, reveals a change from ellipsoid to globular shape of the whole complex arising from a slight compaction of the protein core and an increase of the polymer’s radius of gyration as a function of the grafting polymer density. In particular, we highlight a gradual change of the polymer conformation around the protein and elongation of the semimajor dimension of the ellipsoidal protein. Our results will contribute to the description of biophysical characteristics of a new class of biologically relevant protein–polymer conjugates
536 _ _ |a 6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623)
|0 G:(DE-HGF)POF3-6G4
|c POF3-623
|f POF III
|x 0
536 _ _ |0 G:(DE-HGF)POF3-6G15
|f POF III
|x 1
|c POF3-6G15
|a 6G15 - FRM II / MLZ (POF3-6G15)
588 _ _ |a Dataset connected to CrossRef
650 2 7 |a Soft Condensed Matter
|0 V:(DE-MLZ)SciArea-210
|2 V:(DE-HGF)
|x 0
650 1 7 |a Polymers, Soft Nano Particles and Proteins
|0 V:(DE-MLZ)GC-1602-2016
|2 V:(DE-HGF)
|x 0
693 _ _ |a Forschungs-Neutronenquelle Heinz Maier-Leibnitz
|e KWS-2: Small angle scattering diffractometer
|f NL3ao
|1 EXP:(DE-MLZ)FRMII-20140101
|0 EXP:(DE-MLZ)KWS2-20140101
|5 EXP:(DE-MLZ)KWS2-20140101
|6 EXP:(DE-MLZ)NL3ao-20140101
|x 0
700 1 _ |a de Angelis, Andrea
|0 P:(DE-HGF)0
|b 1
700 1 _ |a Garvey, Christopher. J.
|0 P:(DE-HGF)0
|b 2
700 1 _ |a Wurm, Frederick R.
|0 0000-0002-6955-8489
|b 3
700 1 _ |a Appavou, Marie-Sousai
|0 P:(DE-Juel1)130507
|b 4
|u fzj
700 1 _ |a Prevost, Sylvain
|0 P:(DE-HGF)0
|b 5
773 _ _ |a 10.1021/acs.biomac.9b00184
|g Vol. 20, no. 5, p. 1944 - 1955
|0 PERI:(DE-600)2006291-6
|n 5
|p 1944 - 1955
|t Biomacromolecules
|v 20
|y 2019
|x 1526-4602
856 4 _ |u https://juser.fz-juelich.de/record/867572/files/acs.biomac.9b00184-1.pdf
|y Restricted
856 4 _ |u https://juser.fz-juelich.de/record/867572/files/acs.biomac.9b00184-1.pdf?subformat=pdfa
|x pdfa
|y Restricted
909 C O |o oai:juser.fz-juelich.de:867572
|p VDB:MLZ
|p VDB
910 1 _ |a External Institute
|0 I:(DE-HGF)0
|k Extern
|b 0
|6 0000-0001-6625-2284
910 1 _ |a External Institute
|0 I:(DE-HGF)0
|k Extern
|b 1
|6 P:(DE-HGF)0
910 1 _ |a External Institute
|0 I:(DE-HGF)0
|k Extern
|b 2
|6 P:(DE-HGF)0
910 1 _ |a Forschungszentrum Jülich
|0 I:(DE-588b)5008462-8
|k FZJ
|b 4
|6 P:(DE-Juel1)130507
910 1 _ |a External Institute
|0 I:(DE-HGF)0
|k Extern
|b 5
|6 P:(DE-HGF)0
913 1 _ |a DE-HGF
|b Forschungsbereich Materie
|l Von Materie zu Materialien und Leben
|1 G:(DE-HGF)POF3-620
|0 G:(DE-HGF)POF3-623
|2 G:(DE-HGF)POF3-600
|v Facility topic: Neutrons for Research on Condensed Matter
|9 G:(DE-HGF)POF3-6G4
|x 0
|4 G:(DE-HGF)POF
|3 G:(DE-HGF)POF3
913 1 _ |a DE-HGF
|9 G:(DE-HGF)POF3-6G15
|x 1
|4 G:(DE-HGF)POF
|v FRM II / MLZ
|1 G:(DE-HGF)POF3-6G0
|0 G:(DE-HGF)POF3-6G15
|3 G:(DE-HGF)POF3
|2 G:(DE-HGF)POF3-600
|b Forschungsbereich Materie
|l Großgeräte: Materie
914 1 _ |y 2019
915 _ _ |a JCR
|0 StatID:(DE-HGF)0100
|2 StatID
|b BIOMACROMOLECULES : 2017
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0200
|2 StatID
|b SCOPUS
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0300
|2 StatID
|b Medline
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0310
|2 StatID
|b NCBI Molecular Biology Database
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0600
|2 StatID
|b Ebsco Academic Search
915 _ _ |a Peer Review
|0 StatID:(DE-HGF)0030
|2 StatID
|b ASC
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0199
|2 StatID
|b Clarivate Analytics Master Journal List
915 _ _ |a WoS
|0 StatID:(DE-HGF)0110
|2 StatID
|b Science Citation Index
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0150
|2 StatID
|b Web of Science Core Collection
915 _ _ |a WoS
|0 StatID:(DE-HGF)0111
|2 StatID
|b Science Citation Index Expanded
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1030
|2 StatID
|b Current Contents - Life Sciences
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1150
|2 StatID
|b Current Contents - Physical, Chemical and Earth Sciences
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1050
|2 StatID
|b BIOSIS Previews
915 _ _ |a IF >= 5
|0 StatID:(DE-HGF)9905
|2 StatID
|b BIOMACROMOLECULES : 2017
920 _ _ |l yes
920 1 _ |0 I:(DE-Juel1)JCNS-FRM-II-20110218
|k JCNS-FRM-II
|l JCNS-FRM-II
|x 0
920 1 _ |0 I:(DE-Juel1)JCNS-1-20110106
|k JCNS-1
|l Neutronenstreuung
|x 1
920 1 _ |0 I:(DE-588b)4597118-3
|k MLZ
|l Heinz Maier-Leibnitz Zentrum
|x 2
980 _ _ |a journal
980 _ _ |a VDB
980 _ _ |a I:(DE-Juel1)JCNS-FRM-II-20110218
980 _ _ |a I:(DE-Juel1)JCNS-1-20110106
980 _ _ |a I:(DE-588b)4597118-3
980 _ _ |a UNRESTRICTED

LibraryCollectionCLSMajorCLSMinorLanguageAuthor
Marc 21