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@ARTICLE{Claaen:867614,
      author       = {Claaßen, Christiane and Mack, Kevin and Rother, Dörte},
      title        = {{B}enchtop {NMR} for online reaction monitoring of the
                      biocatalytic synthesis of aromatic amino alcohols},
      journal      = {ChemCatChem},
      volume       = {12},
      number       = {4},
      issn         = {1867-3880},
      address      = {Weinheim},
      publisher    = {WILEY-VCH Verlag},
      reportid     = {FZJ-2019-06235},
      pages        = {1190-1199},
      year         = {2020},
      abstract     = {Online analytics provides insights into the progress of an
                      ongoing reaction without the need for extensive sampling and
                      offline analysis. In this study, we investigated benchtop
                      NMR as an online reaction monitoring tool for complex enzyme
                      cascade reactions. Online NMR was used to monitor a
                      two‐step cascade beginning with an aromatic aldehyde and
                      leading to an aromatic amino alcohol as the final product,
                      applying two different enzymes and a variety of
                      co‐substrates and intermediates. Benchtop NMR enabled the
                      concentration of the reaction components to be detected in
                      buffered systems in the single‐digit mM range without
                      using deuterated solvent. The concentrations determined via
                      NMR were correlated with offline samples analyzed via uHPLC
                      and displayed a good correlation between the two methods. In
                      summary, benchtop NMR proved to be a sensitive, selective
                      and reliable method for online reaction monitoring in
                      (multi‐step) biosynthesis. In future, online analytic
                      systems such as the benchtop NMR devices described might not
                      only enable direct monitoring of the reaction, but may also
                      form the basis for self‐regulation in biocatalytic
                      reactions.},
      cin          = {IBG-1},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000509320100001},
      doi          = {10.1002/cctc.201901910},
      url          = {https://juser.fz-juelich.de/record/867614},
}