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@ARTICLE{ReineckeLevi:868044,
      author       = {Reinecke-Levi, Diana and Castillo-Flores, Antonio and
                      Boussiba, Sammy and Zarka, Aliza},
      title        = {{P}olyploid polynuclear consecutive cell-cycle enables
                      large genome-size in {H}aematococcus pluvialis},
      journal      = {Algal Research},
      volume       = {33},
      issn         = {2211-9264},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier},
      reportid     = {FZJ-2019-06637},
      pages        = {456 - 461},
      year         = {2018},
      abstract     = {Studies revealed and keep revealing a staggering diversity
                      of life-cycle patterns and genome-sizes in microalgae. Even
                      within a single order like the Volvocales with Volvox
                      carteri, Chlamydomonas reinhardtii, and Dunaliella salina,
                      differences are significant. Additional knowledge gaps in
                      microalgal physiology and genetics hinder our understanding
                      of their evolution and valorization. Herein, we present for
                      the first time, the multiple fission cell-cycle with
                      corresponding DNA quantities of the economically important
                      Volvocales Haematococcus pluvialis. Further, we provide two
                      straightforward protocols for efficient culture
                      synchronization, based on filtration or skimming of
                      zoospores. The cell-cycle and nuclear DNA content where
                      monitored by flow cytometry and fluorescence microscopy
                      after staining with dsDNA dye PICO- or SYBR-green®. The
                      average nuclear DNA content was 3.6–4.1 and
                      22.6–25.2 pg in zoospores/coenobial daughter-cells and
                      aplanospores, respectively. We found a single DNA
                      replication step for all coenobial daughter cells prior
                      mitosis, followed by one consecutive cytokinesis. Thus, our
                      results suggest a new Haematococcus-type Cn cell-cycle with
                      unusual polyploid and polynuclear phases. This cell-cycle
                      regime might allow for the large genome-size of
                      Haematococcus and is consistent with the mutational-hazard
                      hypothesis for Volvocales microalgae.},
      cin          = {IBG-2},
      ddc          = {580},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {582 - Plant Science (POF3-582)},
      pid          = {G:(DE-HGF)POF3-582},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000438910600052},
      doi          = {10.1016/j.algal.2018.06.013},
      url          = {https://juser.fz-juelich.de/record/868044},
}