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| Hauptseite > Publikationsdatenbank > Dimerization energetics of the G‐protein coupled bile acid receptor TGR5 from all‐atom simulations > print |
| Hauptseite > Publikationsdatenbank > Dimerization energetics of the G‐protein coupled bile acid receptor TGR5 from all‐atom simulations > print |
| 001 | 868376 | ||
| 005 | 20230815122838.0 | ||
| 024 | 7 | _ | |a 10.1002/jcc.26135 |2 doi |
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| 024 | 7 | _ | |a 1096-987X |2 ISSN |
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| 100 | 1 | _ | |a Wäschenbach, Lucas |0 0000-0003-0044-4552 |b 0 |
| 245 | _ | _ | |a Dimerization energetics of the G‐protein coupled bile acid receptor TGR5 from all‐atom simulations |
| 260 | _ | _ | |a New York, NY [u.a.] |c 2020 |b Wiley |
| 336 | 7 | _ | |a article |2 DRIVER |
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| 520 | _ | _ | |a We describe the first extensive energetic evaluation of GPCR dimerization on the atomistic level by means of potential of mean force (PMF) computations and implicit solvent/implicit membrane end‐point free energy calculations (MM‐PBSA approach). Free energies of association computed from the PMFs show that the formation of both the 1/8 and 4/5 interface is energetically favorable for TGR5, the first GPCR known to be activated by hydrophobic bile acids and neurosteroids. Furthermore, formation of the 1/8 interface is favored over that of the 4/5 interface. Both results are in line with our previous FRET experiments in live cells. Differences in lipid‐protein interactions are identified to contribute to the observed differences in free energies of association. A per‐residue decomposition of the MM‐PBSA effective binding energy reveals hot spot residues specific for both interfaces that form clusters. This knowledge may be used to guide the design of dimerization inhibitors or perform mutational studies to explore physiological consequences of distorted TGR5 association. Finally, we characterized the role of Y111, located in the conserved (D/E)RY motif, as a facilitator of TGR5 interactions. The types of computations performed here should be transferable to other transmembrane proteins that form dimers or higher oligomers as long as good structural models of the dimeric or oligomeric states are available. Such computations may help to overcome current restrictions due to an imperfect energetic representation of protein association at the coarse‐grained level. |
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| 536 | _ | _ | |a Antagonists of the TGR5 G-protein complex formation (hdd15_20170501) |0 G:(DE-Juel1)hdd15_20170501 |c hdd15_20170501 |x 2 |f Antagonists of the TGR5 G-protein complex formation |
| 536 | _ | _ | |a Energetics of the dimerization and G-protein coupling of the bile-acid sensing GPCR TGR5 (hdd15_20160501) |0 G:(DE-Juel1)hdd15_20160501 |c hdd15_20160501 |x 3 |f Energetics of the dimerization and G-protein coupling of the bile-acid sensing GPCR TGR5 |
| 536 | _ | _ | |a DFG project 190586431 - SFB 974: Kommunikation und Systemrelevanz bei Leberschädigung und Regeneration |0 G:(GEPRIS)190586431 |c 190586431 |x 4 |
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| 700 | 1 | _ | |a Keitel, Verena |0 P:(DE-HGF)0 |b 2 |
| 700 | 1 | _ | |a Gohlke, Holger |0 P:(DE-Juel1)172663 |b 3 |e Corresponding author |
| 773 | _ | _ | |a 10.1002/jcc.26135 |g p. jcc.26135 |0 PERI:(DE-600)1479181-x |n 9 |p 874-884 |t Journal of computational chemistry |v 41 |y 2020 |x 1096-987X |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/868376/files/W-schenbach_et_al-2020-Journal_of_Computational_Chemistry.pdf |y Restricted |
| 856 | 4 | _ | |y Published on 2019-12-27. Available in OpenAccess from 2020-12-27. |u https://juser.fz-juelich.de/record/868376/files/Energetics_of_TGR5_dimerization_manuscript_rev.pdf |
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