TY  - JOUR
AU  - Becker, Johanna
AU  - Hosseinpour Tehrani, Hamed
AU  - Gauert, Marc
AU  - Mampel, Jörg
AU  - Blank, Lars M.
AU  - Wierckx, Nick
TI  - An Ustilago maydis chassis for itaconic acid production without by‐products
JO  - Microbial biotechnology
VL  - 13
IS  - 2
SN  - 1751-7915
CY  - Oxford
PB  - Wiley-Blackwell
M1  - FZJ-2020-00059
SP  - 350 - 362
PY  - 2020
N1  - Biotechnologie 1
AB  - Ustilago maydis is a promising yeast for the production of a range of valuable metabolites, including itaconate, malate, glycolipids and triacylglycerols. However, wild‐type strains generally produce a potpourri of all of these metabolites, which hinders efficient production of single target chemicals. In this study, the diverse by‐product spectrum of U. maydis was reduced through strain engineering using CRISPR/Cas9 and FLP/FRT, greatly increasing the metabolic flux into the targeted itaconate biosynthesis pathway. With this strategy, a marker‐free chassis strain could be engineered, which produces itaconate from glucose with significantly enhanced titre, rate and yield. The lack of by‐product formation not only benefited itaconate production, it also increases the efficiency of downstream processing improving cell handling and product purity.
LB  - PUB:(DE-HGF)16
C6  - pmid:31880860
UR  - <Go to ISI:>//WOS:000504538600001
DO  - DOI:10.1111/1751-7915.13525
UR  - https://juser.fz-juelich.de/record/872541
ER  -