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@ARTICLE{Becker:872541,
      author       = {Becker, Johanna and Hosseinpour Tehrani, Hamed and Gauert,
                      Marc and Mampel, Jörg and Blank, Lars M. and Wierckx, Nick},
      title        = {{A}n {U}stilago maydis chassis for itaconic acid production
                      without by‐products},
      journal      = {Microbial biotechnology},
      volume       = {13},
      number       = {2},
      issn         = {1751-7915},
      address      = {Oxford},
      publisher    = {Wiley-Blackwell},
      reportid     = {FZJ-2020-00059},
      pages        = {350 - 362},
      year         = {2020},
      note         = {Biotechnologie 1},
      abstract     = {Ustilago maydis is a promising yeast for the production of
                      a range of valuable metabolites, including itaconate,
                      malate, glycolipids and triacylglycerols. However,
                      wild‐type strains generally produce a potpourri of all of
                      these metabolites, which hinders efficient production of
                      single target chemicals. In this study, the diverse
                      by‐product spectrum of U. maydis was reduced through
                      strain engineering using CRISPR/Cas9 and FLP/FRT, greatly
                      increasing the metabolic flux into the targeted itaconate
                      biosynthesis pathway. With this strategy, a marker‐free
                      chassis strain could be engineered, which produces itaconate
                      from glucose with significantly enhanced titre, rate and
                      yield. The lack of by‐product formation not only benefited
                      itaconate production, it also increases the efficiency of
                      downstream processing improving cell handling and product
                      purity.},
      cin          = {IBG-1},
      ddc          = {610},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31880860},
      UT           = {WOS:000504538600001},
      doi          = {10.1111/1751-7915.13525},
      url          = {https://juser.fz-juelich.de/record/872541},
}