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@ARTICLE{Solovchenko:872659,
      author       = {Solovchenko, Alexei and Khozin-Goldberg, Inna and Selyakh,
                      Irina and Semenova, Larisa and Ismagulova, Tatiana and
                      Lukyanov, Alexandr and Mamedov, Ilgar and Vinogradova,
                      Elizaveta and Karpova, Olga and Konyukhov, Ivan and
                      Vasilieva, Svetlana and Mojzes, Peter and Dijkema, Cor and
                      Vecherskaya, Margarita and Zvyagin, Ivan and Nedbal,
                      Ladislav and Gorelova, Olga},
      title        = {{P}hosphorus starvation and luxury uptake in green
                      microalgae revisited},
      journal      = {Algal Research},
      volume       = {43},
      issn         = {2211-9264},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier},
      reportid     = {FZJ-2020-00150},
      pages        = {101651 -},
      year         = {2019},
      abstract     = {Phosphorus (P) is central to storing and transferring
                      energy and information in living cells, including those of
                      microalgae. Many microalgal species dwelling in low P
                      environments are naturally equipped to take up and store P
                      whenever it becomes available through a complex phenomenon
                      known as “luxury P uptake.” Its research is required for
                      better understanding of the nutrient geochemical cycles in
                      aquatic environments but also for biotechnological
                      applications such as sequestration of nutrients from
                      wastewater and production of algal fertilizers. Here, we
                      report on our recent insights into luxury P uptake and
                      polyphosphate formation originating from physiological,
                      ultrastructural, and transcriptomic evidence. The cultures
                      pre-starved of P and re-fed with inorganic phosphate (Pi)
                      exhibited a bi-phasic kinetics of Pi uptake comprising fast
                      (1–2 h after re-feeding) and slow (1–3 d after
                      re-feeding) phases. The rate of Pi uptake in the fast phase
                      was ca. 10 times higher than in the slow phase with an
                      opposite trend shown for the cell division rate. The
                      transient peak of polyphosphate accumulation was determined
                      2–4 h after re-feeding and coincided with the period of
                      slow cell division and fast Pi uptake. In this phase, the
                      microalgal cells reached the highest P content (up to $5\%$
                      of dry cell weight). The P re-feeding also reversed the
                      characteristic changes in cell lipids induced by P
                      starvation, namely increase in the major membrane glycolipid
                      (DGDG/MGDG) ratio and betaine lipids. These changes were
                      reversed upon Pi re-feeding of the starved culture. Electron
                      microscopy revealed the ordered organization of vacuolar
                      polyphosphate indicative of the possible involvement of an
                      enzyme (complex) in their synthesis. A candidate gene
                      encoding a protein similar to the vacuolar transport
                      chaperone (VTC) protein, featuring an expression pattern
                      corresponding to polyphosphate accumulation, was revealed.
                      Implications of the findings for efficient biocapture of
                      phosphorus are discussed.},
      cin          = {IBG-2},
      ddc          = {580},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {582 - Plant Science (POF3-582)},
      pid          = {G:(DE-HGF)POF3-582},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000489307800025},
      doi          = {10.1016/j.algal.2019.101651},
      url          = {https://juser.fz-juelich.de/record/872659},
}