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000872749 1001_ $$0P:(DE-Juel1)168161$$aZhang, Yuting$$b0$$ufzj
000872749 245__ $$aAmperometric Aptasensor for Amyloid-β Oligomer Detection by Optimized Stem-Loop Structures with an Adjustable Detection Range
000872749 260__ $$aWashington, DC$$bACS Publications$$c2019
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000872749 520__ $$aAmyloid-β oligomers (AβO) have become representative biomarkers for early diagnosis of Alzheimer's disease. Here, we report on an aptasensor based on stem-loop probes for sensitive and specific detection of AβO by an amperometric transducer principle using alternating current voltammetry (ACV). Stem-loop probes with redox-active moieties are immobilized on a gold substrate as a receptor element. The signal transduction mechanism relies on redox ferrocene (Fc) reporting via charge transfer on a molecular recognition event involving a conformational change of the molecular beacon. The stem-loop structures were optimized by considering the aptamers' stem length, spacer, and different ferrocene terminals. In addition, the sensor assembly and signal recording including aptamer concentration and ACV frequency dependence are discussed. Using the optimized stem-loop probe (B-3' Fc), the aptasensor showed a decrease of the Fc peak current induced by AβO binding within the broad concentration range spanning 6 orders of magnitude. Furthermore, the detection limit of the sensor can be further decreased by optimizing the ACV frequency, however at the cost of a narrowed detection range. In this work, a label-free electrochemical aptasensor is demonstrated, which facilitates the quantification of the concentration of AβO with high selectivity and subpicomolar sensitivity, which may be conducive to improving the diagnosis and pharmacology studies of Alzheimer's disease.
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000872749 7001_ $$0P:(DE-HGF)0$$aFigueroa-Miranda, Gabriela$$b1
000872749 7001_ $$0P:(DE-Juel1)162137$$aZafiu, Christian$$b2$$ufzj
000872749 7001_ $$0P:(DE-Juel1)132029$$aWillbold, Dieter$$b3
000872749 7001_ $$0P:(DE-Juel1)128713$$aOffenhäusser, Andreas$$b4
000872749 7001_ $$0P:(DE-Juel1)128707$$aMayer, Dirk$$b5$$eCorresponding author
000872749 773__ $$0PERI:(DE-600)2843497-3$$a10.1021/acssensors.9b01630$$gVol. 4, no. 11, p. 3042 - 3050$$n11$$p3042 - 3050$$tACS sensors$$v4$$x2379-3694$$y2019
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000872749 8564_ $$uhttps://juser.fz-juelich.de/record/872749/files/Autorenmanuskript.pdf$$yPublished on 2019-11-01. Available in OpenAccess from 2020-11-01.
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