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@ARTICLE{Zhang:872749,
      author       = {Zhang, Yuting and Figueroa-Miranda, Gabriela and Zafiu,
                      Christian and Willbold, Dieter and Offenhäusser, Andreas
                      and Mayer, Dirk},
      title        = {{A}mperometric {A}ptasensor for {A}myloid-β {O}ligomer
                      {D}etection by {O}ptimized {S}tem-{L}oop {S}tructures with
                      an {A}djustable {D}etection {R}ange},
      journal      = {ACS sensors},
      volume       = {4},
      number       = {11},
      issn         = {2379-3694},
      address      = {Washington, DC},
      publisher    = {ACS Publications},
      reportid     = {FZJ-2020-00227},
      pages        = {3042 - 3050},
      year         = {2019},
      abstract     = {Amyloid-β oligomers (AβO) have become representative
                      biomarkers for early diagnosis of Alzheimer's disease. Here,
                      we report on an aptasensor based on stem-loop probes for
                      sensitive and specific detection of AβO by an amperometric
                      transducer principle using alternating current voltammetry
                      (ACV). Stem-loop probes with redox-active moieties are
                      immobilized on a gold substrate as a receptor element. The
                      signal transduction mechanism relies on redox ferrocene (Fc)
                      reporting via charge transfer on a molecular recognition
                      event involving a conformational change of the molecular
                      beacon. The stem-loop structures were optimized by
                      considering the aptamers' stem length, spacer, and different
                      ferrocene terminals. In addition, the sensor assembly and
                      signal recording including aptamer concentration and ACV
                      frequency dependence are discussed. Using the optimized
                      stem-loop probe (B-3' Fc), the aptasensor showed a decrease
                      of the Fc peak current induced by AβO binding within the
                      broad concentration range spanning 6 orders of magnitude.
                      Furthermore, the detection limit of the sensor can be
                      further decreased by optimizing the ACV frequency, however
                      at the cost of a narrowed detection range. In this work, a
                      label-free electrochemical aptasensor is demonstrated, which
                      facilitates the quantification of the concentration of AβO
                      with high selectivity and subpicomolar sensitivity, which
                      may be conducive to improving the diagnosis and pharmacology
                      studies of Alzheimer's disease.},
      cin          = {ICS-6},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ICS-6-20110106},
      pnm          = {553 - Physical Basis of Diseases (POF3-553)},
      pid          = {G:(DE-HGF)POF3-553},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31674772},
      UT           = {WOS:000499737100028},
      doi          = {10.1021/acssensors.9b01630},
      url          = {https://juser.fz-juelich.de/record/872749},
}