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000872750 1001_ $$0P:(DE-Juel1)141850$$aKrichel, Carsten$$b0
000872750 245__ $$aSolution structure of the autophagy-related protein LC3C reveals a polyproline II motif on a mobile tether with phosphorylation site
000872750 260__ $$a[London]$$bMacmillan Publishers Limited, part of Springer Nature$$c2019
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000872750 520__ $$a(Macro-)autophagy is a compartmental degradation pathway conserved from yeast to mammals. The yeast protein Atg8 mediates membrane tethering/hemifusion and cargo recruitment and is essential for autophagy. The human MAP1LC3/GABARAP family proteins show high sequence identity with Atg8, but MAP1LC3C is distinguished by a conspicuous amino-terminal extension with unknown functional significance. We have determined the high-resolution three-dimensional structure and measured the backbone dynamics of MAP1LC3C by NMR spectroscopy. From Ser18 to Ala120, MAP1LC3C forms an α-helix followed by the ubiquitin-like tertiary fold with two hydrophobic binding pockets used by MAP1LC3/GABARAP proteins to recognize targets presenting LC3-interacting regions (LIRs). Unlike other MAP1LC3/GABARAP proteins, the amino-terminal region of MAP1LC3C does not form a stable helix α1 but a “sticky arm” consisting of a polyproline II motif on a flexible linker. Ser18 at the interface between this linker and the structural core can be phosphorylated in vitro by protein kinase A, which causes additional conformational heterogeneity as monitored by NMR spectroscopy and molecular dynamics simulations, including changes in the LIR-binding interface. Based on these results we propose that the amino-terminal polyproline II motif mediates specific interactions with the microtubule cytoskeleton and that Ser18 phosphorylation modulates the interplay of MAP1LC3C with its various target proteins.
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000872750 7001_ $$0P:(DE-Juel1)169918$$aMöckel, Christina$$b1$$ufzj
000872750 7001_ $$0P:(DE-Juel1)157850$$aSchillinger, Oliver$$b2
000872750 7001_ $$0P:(DE-Juel1)162356$$aHuesgen, Pitter F.$$b3
000872750 7001_ $$0P:(DE-HGF)0$$aSticht, Heinrich$$b4
000872750 7001_ $$0P:(DE-Juel1)132024$$aStrodel, Birgit$$b5
000872750 7001_ $$0P:(DE-Juel1)131988$$aWeiergräber, Oliver H.$$b6$$ufzj
000872750 7001_ $$0P:(DE-Juel1)132029$$aWillbold, Dieter$$b7$$eCorresponding author
000872750 7001_ $$0P:(DE-Juel1)144510$$aNeudecker, Philipp$$b8$$eCorresponding author
000872750 773__ $$0PERI:(DE-600)2615211-3$$a10.1038/s41598-019-48155-8$$gVol. 9, no. 1, p. 14167$$n1$$p14167$$tScientific reports$$v9$$x2045-2322$$y2019
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