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@ARTICLE{Perov:872752,
author = {Perov, Sergei and Lidor, Ofir and Salinas, Nir and Golan,
Nimrod and Tayeb- Fligelman, Einav and Deshmukh, Maya and
Willbold, Dieter and Landau, Meytal},
title = {{S}tructural {I}nsights into {C}urli {C}sg{A} {C}ross-β
{F}ibril {A}rchitecture {I}nspire {R}epurposing of
{A}nti-amyloid {C}ompounds as {A}nti-biofilm {A}gents},
journal = {PLoS pathogens},
volume = {15},
number = {8},
issn = {1553-7374},
address = {Lawrence, Kan.},
publisher = {PLoS},
reportid = {FZJ-2020-00230},
pages = {e1007978 -},
year = {2019},
abstract = {Curli amyloid fibrils secreted by Enterobacteriaceae
mediate host cell adhesion and contribute to biofilm
formation, thereby promoting bacterial resistance to
environmental stressors. Here, we present crystal structures
of amyloid-forming segments from the major curli subunit,
CsgA, revealing steric zipper fibrils of tightly mated
β-sheets, demonstrating a structural link between curli and
human pathological amyloids. D-enantiomeric peptides,
originally developed to interfere with Alzheimer's
disease-associated amyloid-β, inhibited CsgA fibrillation
and reduced biofilm formation in Salmonella typhimurium.
Moreover, as previously shown, CsgA fibrils cross-seeded
fibrillation of amyloid-β, providing support for the
proposed structural resemblance and potential for
cross-species amyloid interactions. The presented findings
provide structural insights into amyloidogenic regions
important for curli formation, suggest a novel strategy for
disrupting amyloid-structured biofilms, and hypothesize on
the formation of self-propagating prion-like species
originating from a microbial source that could influence
neurodegenerative diseases},
cin = {ICS-6},
ddc = {610},
cid = {I:(DE-Juel1)ICS-6-20110106},
pnm = {553 - Physical Basis of Diseases (POF3-553)},
pid = {G:(DE-HGF)POF3-553},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:31469892},
UT = {WOS:000488322100029},
doi = {10.1371/journal.ppat.1007978},
url = {https://juser.fz-juelich.de/record/872752},
}