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@ARTICLE{Alvarez:872761,
author = {Alvarez, Clarisa E. and Bovdilova, Anastasiia and Höppner,
Astrid and Wolff, Christian-Claus and Saigo, Mariana and
Trajtenberg, Felipe and Zhang, Tao and Buschiazzo, Alejandro
and Nagel-Steger, Luitgard and Drincovich, Maria F. and
Lercher, Martin J. and Maurino, Veronica G.},
title = {{M}olecular adaptations of {NADP}-malic enzyme for its
function in {C}4 photosynthesis in grasses},
journal = {Nature plants},
volume = {5},
number = {7},
issn = {2055-0278},
address = {London},
publisher = {Nature Publ. Group},
reportid = {FZJ-2020-00239},
pages = {755 - 765},
year = {2019},
abstract = {In C4 grasses of agronomical interest, malate shuttled into
the bundle sheath cells is decarboxylated mainly by
nicotinamide adenine dinucleotide phosphate (NADP)-malic
enzyme (C4-NADP-ME). The activity of C4-NADP-ME was
optimized by natural selection to efficiently deliver CO2 to
Rubisco. During its evolution from a plastidic
non-photosynthetic NADP-ME, C4-NADP-ME acquired increased
catalytic efficiency, tetrameric structure and pH-dependent
inhibition by its substrate malate. Here, we identified
specific amino acids important for these C4 adaptions based
on strict differential conservation of amino acids, combined
with solving the crystal structures of maize and sorghum
C4-NADP-ME. Site-directed mutagenesis and struc-tural
analyses show that Q503, L544 and E339 are involved in
catalytic efficiency; E339 confers pH-dependent regulation
by malate, F140 is critical for the stabilization of the
oligomeric structure and the N-terminal region is involved
in tetramerization. Together, the identified molecular
adaptations form the basis for the efficient catalysis and
regulation of one of the central biochemical steps in C4
metabolism},
cin = {ICS-6},
ddc = {580},
cid = {I:(DE-Juel1)ICS-6-20110106},
pnm = {552 - Engineering Cell Function (POF3-552)},
pid = {G:(DE-HGF)POF3-552},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:31235877},
UT = {WOS:000474454600022},
doi = {10.1038/s41477-019-0451-7},
url = {https://juser.fz-juelich.de/record/872761},
}