TY  - JOUR
AU  - Spielmann, Alina
AU  - Brack, Yannik
AU  - van Beek, Hugo
AU  - Flachbart, Lion
AU  - Sundermeyer, Lea
AU  - Baumgart, Meike
AU  - Bott, Michael
TI  - NADPH biosensor-based identification of an alcohol dehydrogenase variant with improved catalytic properties caused by a single charge reversal at the protein surface
JO  - AMB express
VL  - 10
IS  - 1
SN  - 2191-0855
CY  - Heidelberg
PB  - Springer
M1  - FZJ-2020-00399
SP  - 14
PY  - 2020
N1  - Biotechnologie 1
AB  - Alcohol dehydrogenases (ADHs) are used in reductive biotransformations for the production of valuable chiral alcohols. In this study, we used a high-throughput screening approach based on the NADPH biosensor pSenSox and fluorescence-activated cell sorting (FACS) to search for variants of the NADPH-dependent ADH of Lactobacillus brevis (LbADH) with improved activity for the reduction of 2,5-hexanedione to (2R,5R)-hexanediol. In a library of approx. 1.4 × 106 clones created by random mutagenesis we identified the variant LbADHK71E. Kinetic analysis of the purified enzyme revealed that LbADHK71E had a ~ 16% lowered KM value and a 17% higher Vmax for 2,5-hexanedione compared to the wild-type LbADH. Higher activities were also observed for the alternative substrates acetophenone, acetylpyridine, 2-hexanone, 4-hydroxy-2-butanone, and methyl acetoacetate. K71 is solvent-exposed on the surface of LbADH and not located within or close to the active site. Therefore, K71 is not an obvious target for rational protein engineering. The study demonstrates that high-throughput screening using the NADPH biosensor pSenSox represents a powerful method to find unexpected beneficial mutations in NADPH-dependent alcohol dehydrogenases that can be favorable in industrial biotransformations.
LB  - PUB:(DE-HGF)16
C6  - pmid:31955268
UR  - <Go to ISI:>//WOS:000514581800005
DO  - DOI:10.1186/s13568-020-0946-7
UR  - https://juser.fz-juelich.de/record/872939
ER  -