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@ARTICLE{Jurischka:872957,
      author       = {Jurischka, Sarah and Bida, Astrid and Dohmen-Olma, Doris
                      and Kleine, Britta and Potzkei, Janko and Binder, Stephan
                      and Schaumann, Georg and Bakkes, Patrick J. and Freudl,
                      Roland},
      title        = {{A} secretion biosensor for monitoring {S}ec-dependent
                      protein export in {C}orynebacterium glutamicum},
      journal      = {Microbial cell factories},
      volume       = {19},
      number       = {1},
      issn         = {1475-2859},
      address      = {London},
      publisher    = {Biomed Central},
      reportid     = {FZJ-2020-00417},
      pages        = {11},
      year         = {2020},
      note         = {Biotechnologie 1},
      abstract     = {BackgroundIn recent years, the industrial workhorse
                      Corynebacterium glutamicum has gained increasing interest as
                      a host organism for the secretory production of heterologous
                      proteins. Generally, the yield of a target protein in the
                      culture supernatant depends on a multitude of interdependent
                      biological and bioprocess parameters which have to be
                      optimized. So far, the monitoring of such optimization
                      processes depends on the availability of a direct assay for
                      the respective target protein that can be handled also in
                      high throughput approaches. Since simple assays, such as
                      standard enzymatic activity assays, are not always at hand,
                      the availability of a general protein secretion biosensor is
                      highly desirable.ResultsHigh level secretion of proteins via
                      the Sec protein export pathway leads to secretion stress, a
                      phenomenon that is thought to be caused by the accumulation
                      of incompletely or misfolded proteins at the membrane-cell
                      envelope interface. We have analyzed the transcriptional
                      responses of C. glutamicum to the secretory production of
                      two different heterologous proteins and found that, in both
                      cases, the expression of the gene encoding a homologue of
                      the extracytosolic HtrA protease was highly upregulated.
                      Based on this finding, a C. glutamicum Sec secretion
                      biosensor strain was constructed in which the htrA gene on
                      the chromosome was replaced by the eyfp gene. The
                      fluorescence of the resulting reporter strain responded to
                      the secretion of different heterologous proteins (cutinase
                      from Fusarium solani pisi and alkaline phosphatase PhoA from
                      Escherichia coli) in a dose-dependent manner. In addition,
                      three differently efficient signal peptides for the
                      secretory production of the cutinase could be differentiated
                      by the biosensor signal. Furthermore, we have shown that an
                      efficient signal peptide can be separated from a poor signal
                      peptide by using the biosensor signal of the respective
                      cells in fluorescence activated cell sorting
                      experiments.ConclusionsWe have succeeded in the construction
                      of a C. glutamicum biosensor strain that allows for the
                      monitoring of Sec-dependent secretion of heterologous
                      proteins in a dose-dependent manner, independent of a direct
                      assay for the desired target protein.},
      cin          = {IBG-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31964372},
      UT           = {WOS:000521188600001},
      doi          = {10.1186/s12934-019-1273-z},
      url          = {https://juser.fz-juelich.de/record/872957},
}