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@ARTICLE{Niether:873342,
      author       = {Niether, Doreen and Sarter, Mona and Koenig, Bernd W. and
                      Fitter, Jörg and Stadler, Andreas M. and Wiegand, Simone},
      title        = {{T}hermophoresis: {T}he {C}ase of {S}treptavidin and
                      {B}iotin},
      journal      = {Polymers},
      volume       = {12},
      number       = {2},
      issn         = {2073-4360},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {FZJ-2020-00655},
      pages        = {376 -},
      year         = {2020},
      abstract     = {Thermophoretic behavior of a free protein changes upon
                      ligand binding and gives access to information on the
                      binding constants. The Soret effect has also been proven to
                      be a promising tool to gain information on the hydration
                      layer, as the temperature dependence of the thermodiffusion
                      behavior is sensitive to solute–solvent interactions. In
                      this work, we perform systematic thermophoretic measurements
                      of the protein streptavidin (STV) and of the complex STV
                      with biotin (B) using thermal diffusion forced Rayleigh
                      scattering (TDFRS). Our experiments show that the
                      temperature sensitivity of the Soret coefficient is reduced
                      for the complex compared to the free protein. We discuss our
                      data in comparison with recent quasi-elastic neutron
                      scattering (QENS) measurements. As the QENS measurement has
                      been performed in heavy water, we perform additional
                      measurements in water/heavy water mixtures. Finally, we also
                      elucidate the challenges arising from the quantiative
                      thermophoretic study of complex multicomponent systems such
                      as protein solutions.},
      cin          = {IEK-8 / ICS-3 / ICS-5 / ICS-6 / JCNS-1},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IEK-8-20101013 / I:(DE-Juel1)ICS-3-20110106 /
                      I:(DE-Juel1)ICS-5-20110106 / I:(DE-Juel1)ICS-6-20110106 /
                      I:(DE-Juel1)JCNS-1-20110106},
      pnm          = {551 - Functional Macromolecules and Complexes (POF3-551) /
                      EUSMI - European infrastructure for spectroscopy, scattering
                      and imaging of soft matteer (731019) / 243 - Tropospheric
                      trace substances and their transformation processes
                      (POF3-243)},
      pid          = {G:(DE-HGF)POF3-551 / G:(EU-Grant)731019 /
                      G:(DE-HGF)POF3-243},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32046223},
      UT           = {WOS:000519849800126},
      doi          = {10.3390/polym12020376},
      url          = {https://juser.fz-juelich.de/record/873342},
}