000873638 001__ 873638
000873638 005__ 20240619091254.0
000873638 037__ $$aFZJ-2020-00881
000873638 1001_ $$0P:(DE-Juel1)164250$$aHlukhova, Hanna$$b0
000873638 1112_ $$aMRS Fall Meeting$$cBoston$$d2019-12-01 - 2019-12-06$$wUSA
000873638 245__ $$aLabel-free aptamer – based detection of Amyloid Beta proteins on SiO2 covered silicon structures
000873638 260__ $$c2019
000873638 3367_ $$033$$2EndNote$$aConference Paper
000873638 3367_ $$2DataCite$$aOther
000873638 3367_ $$2BibTeX$$aINPROCEEDINGS
000873638 3367_ $$2DRIVER$$aconferenceObject
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000873638 3367_ $$0PUB:(DE-HGF)6$$2PUB:(DE-HGF)$$aConference Presentation$$bconf$$mconf$$s1587365919_5748$$xAfter Call
000873638 520__ $$aNeurodegenerative processes in brain became important challenge with increased age of population. Usually relatively bulky optical methods are used to detect Amyloid β peptides, which are linked to the pathogenesis of Alzheimer disease. To prolong effective and healthy memory and thinking capability as well as to stop the factors resulting in Alzheimer’s disease new high-sensitive early-stage methods for detection of peptides involved in Alzhemer’s disease have to be developed. In this contribution we report on successful demonstration of new label-free approach for an aptamer-based detection of amyloid beta proteins. A high-selective nucleic acid aptamer was synthesized and immobilized onto SiO2 surface for detection of the target Amyloid beta-40 peptide. Functionalized structures on thin film dielectric layer has been developed to detect ultra-low concentrations of target protein. The detection technique utilizes high selection aptamer immobilization on the SiO2 dielectric layer of system configured with a liquid-gate. The introduction of amyloid beta protein to the sensor surface of different concentration in a wide range from 0.1pM to 1uM results in change of surface potential. These experimental data provide a sensitive detection of ultra-low concentrations of an amyloid beta protein and opens perspective for the development of real-time sensors for studies and understanding of different stages of Alzheimer’s disease utilizing nanostructures fabricated on the basis of cost-efficient CMOS compatible technology.Keywords: Alzheimer, Therapeutics, Flat-band Voltage, Biosensor, Aptamer, nucleic acid, Amyloid Beta, Ab-40, Debye length, Immobilization, Silicon Oxide, Thin Films
000873638 536__ $$0G:(DE-HGF)POF3-523$$a523 - Controlling Configuration-Based Phenomena (POF3-523)$$cPOF3-523$$fPOF III$$x0
000873638 7001_ $$0P:(DE-Juel1)167225$$aKutovyi, Yurii$$b1$$ufzj
000873638 7001_ $$0P:(DE-HGF)0$$aMenger, Markus$$b2
000873638 7001_ $$0P:(DE-Juel1)128713$$aOffenhäusser, Andreas$$b3$$ufzj
000873638 7001_ $$0P:(DE-Juel1)128738$$aVitusevich, Svetlana$$b4$$eCorresponding author$$ufzj
000873638 909CO $$ooai:juser.fz-juelich.de:873638$$pVDB
000873638 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)167225$$aForschungszentrum Jülich$$b1$$kFZJ
000873638 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)128713$$aForschungszentrum Jülich$$b3$$kFZJ
000873638 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)128738$$aForschungszentrum Jülich$$b4$$kFZJ
000873638 9131_ $$0G:(DE-HGF)POF3-523$$1G:(DE-HGF)POF3-520$$2G:(DE-HGF)POF3-500$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bKey Technologies$$lFuture Information Technology - Fundamentals, Novel Concepts and Energy Efficiency (FIT)$$vControlling Configuration-Based Phenomena$$x0
000873638 9141_ $$y2020
000873638 920__ $$lyes
000873638 9201_ $$0I:(DE-Juel1)ICS-8-20110106$$kICS-8$$lBioelektronik$$x0
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000873638 980__ $$aI:(DE-Juel1)ICS-8-20110106
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000873638 981__ $$aI:(DE-Juel1)IBI-3-20200312