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@INPROCEEDINGS{Hlukhova:873638,
      author       = {Hlukhova, Hanna and Kutovyi, Yurii and Menger, Markus and
                      Offenhäusser, Andreas and Vitusevich, Svetlana},
      title        = {{L}abel-free aptamer – based detection of {A}myloid
                      {B}eta proteins on {S}i{O}2 covered silicon structures},
      reportid     = {FZJ-2020-00881},
      year         = {2019},
      abstract     = {Neurodegenerative processes in brain became important
                      challenge with increased age of population. Usually
                      relatively bulky optical methods are used to detect Amyloid
                      β peptides, which are linked to the pathogenesis of
                      Alzheimer disease. To prolong effective and healthy memory
                      and thinking capability as well as to stop the factors
                      resulting in Alzheimer’s disease new high-sensitive
                      early-stage methods for detection of peptides involved in
                      Alzhemer’s disease have to be developed. In this
                      contribution we report on successful demonstration of new
                      label-free approach for an aptamer-based detection of
                      amyloid beta proteins. A high-selective nucleic acid aptamer
                      was synthesized and immobilized onto SiO2 surface for
                      detection of the target Amyloid beta-40 peptide.
                      Functionalized structures on thin film dielectric layer has
                      been developed to detect ultra-low concentrations of target
                      protein. The detection technique utilizes high selection
                      aptamer immobilization on the SiO2 dielectric layer of
                      system configured with a liquid-gate. The introduction of
                      amyloid beta protein to the sensor surface of different
                      concentration in a wide range from 0.1pM to 1uM results in
                      change of surface potential. These experimental data provide
                      a sensitive detection of ultra-low concentrations of an
                      amyloid beta protein and opens perspective for the
                      development of real-time sensors for studies and
                      understanding of different stages of Alzheimer’s disease
                      utilizing nanostructures fabricated on the basis of
                      cost-efficient CMOS compatible technology.Keywords:
                      Alzheimer, Therapeutics, Flat-band Voltage, Biosensor,
                      Aptamer, nucleic acid, Amyloid Beta, Ab-40, Debye length,
                      Immobilization, Silicon Oxide, Thin Films},
      month         = {Dec},
      date          = {2019-12-01},
      organization  = {MRS Fall Meeting, Boston (USA), 1 Dec
                       2019 - 6 Dec 2019},
      subtyp        = {After Call},
      cin          = {ICS-8},
      cid          = {I:(DE-Juel1)ICS-8-20110106},
      pnm          = {523 - Controlling Configuration-Based Phenomena (POF3-523)},
      pid          = {G:(DE-HGF)POF3-523},
      typ          = {PUB:(DE-HGF)6},
      url          = {https://juser.fz-juelich.de/record/873638},
}