000873824 001__ 873824
000873824 005__ 20210130004520.0
000873824 0247_ $$2doi$$a10.3389/fmicb.2020.00087
000873824 0247_ $$2Handle$$a2128/24327
000873824 0247_ $$2altmetric$$aaltmetric:75931298
000873824 0247_ $$2pmid$$apmid:32117117
000873824 0247_ $$2WOS$$aWOS:000517630100001
000873824 037__ $$aFZJ-2020-01029
000873824 082__ $$a570
000873824 1001_ $$0P:(DE-Juel1)171102$$aWolf, Natalie$$b0
000873824 245__ $$aMolecular Basis of Growth Inhibition by Acetate of an Adenylate Cyclase-Deficient Mutant of Corynebacterium glutamicum
000873824 260__ $$aLausanne$$bFrontiers Media$$c2020
000873824 3367_ $$2DRIVER$$aarticle
000873824 3367_ $$2DataCite$$aOutput Types/Journal article
000873824 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1581428743_17239
000873824 3367_ $$2BibTeX$$aARTICLE
000873824 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000873824 3367_ $$00$$2EndNote$$aJournal Article
000873824 500__ $$aBiotechnologie 1
000873824 520__ $$aIn Corynebacterium glutamicum, cyclic adenosine monophosphate (cAMP) serves as an effector of the global transcriptional regulator GlxR. Synthesis of cAMP is catalyzed by the membrane-bound adenylate cyclase CyaB. In this study, we investigated the consequences of decreased intracellular cAMP levels in a ΔcyaB mutant. While no growth defect of the ΔcyaB strain was observed on glucose, fructose, sucrose, or gluconate alone, the addition of acetate to these growth media resulted in a severe growth inhibition, which could be reversed by plasmid-based cyaB expression or by supplementation of the medium with cAMP. The effect was concentration- and pH-dependent, suggesting a link to the uncoupling activity of acetate. In agreement, the ΔcyaB mutant had an increased sensitivity to the protonophore carbonyl cyanide m-chlorophenyl hydrazone (CCCP). The increased uncoupler sensitivity correlated with a lowered membrane potential of acetate-grown ΔcyaB cells compared to wild-type cells. A reduced membrane potential affects major cellular processes, such as ATP synthesis by F1FO-ATP synthase and numerous transport processes. The impaired membrane potential of the ΔcyaB mutant could be due to a decreased expression of the cytochrome bc1-aa3 supercomplex, which is the major contributor of proton-motive force in C. glutamicum. Expression of the supercomplex genes was previously reported to be activated by GlxR-cAMP. A suppressor mutant of the ΔcyaB strain with improved growth on acetate was isolated, which carried a single mutation in the genome leading to an Ala131Thr exchange in GlxR. Introduction of this point mutation into the original ΔcyaB mutant restored the growth defect on acetate. This supported the importance of GlxR for the phenotype of the ΔcyaB mutant and, more generally, of the cAMP-GlxR system for the control of energy metabolism in C. glutamicum.Introduction
000873824 536__ $$0G:(DE-HGF)POF3-581$$a581 - Biotechnology (POF3-581)$$cPOF3-581$$fPOF III$$x0
000873824 588__ $$aDataset connected to CrossRef
000873824 7001_ $$0P:(DE-HGF)0$$aBussmann, Michael$$b1
000873824 7001_ $$0P:(DE-Juel1)128970$$aKoch-Koerfges, Abigail$$b2
000873824 7001_ $$0P:(DE-Juel1)162368$$aKatcharava, Nino$$b3
000873824 7001_ $$0P:(DE-Juel1)159142$$aSchulte, Julia$$b4
000873824 7001_ $$0P:(DE-Juel1)128982$$aPolen, Tino$$b5
000873824 7001_ $$0P:(DE-HGF)0$$aHartl, Johannes$$b6
000873824 7001_ $$0P:(DE-HGF)0$$aVorholt, Julia A.$$b7
000873824 7001_ $$0P:(DE-Juel1)145489$$aBaumgart, Meike$$b8
000873824 7001_ $$0P:(DE-Juel1)128943$$aBott, Michael$$b9$$eCorresponding author
000873824 773__ $$0PERI:(DE-600)2587354-4$$a10.3389/fmicb.2020.00087$$gVol. 11, p. 87$$p87$$tFrontiers in microbiology$$v11$$x1664-302X$$y2020
000873824 8564_ $$uhttps://juser.fz-juelich.de/record/873824/files/Wolf%20et%20al%202020%20with%20supplementary%20data.pdf$$yOpenAccess$$zStatID:(DE-HGF)0510
000873824 8564_ $$uhttps://juser.fz-juelich.de/record/873824/files/Wolf%20et%20al%202020%20Front%20Microbiol%2011%2887%29%20with%20SI%20%28003%29.pdf$$yRestricted$$zStatID:(DE-HGF)0599
000873824 8564_ $$uhttps://juser.fz-juelich.de/record/873824/files/Wolf%20et%20al%202020%20with%20supplementary%20data.pdf?subformat=pdfa$$xpdfa$$yOpenAccess$$zStatID:(DE-HGF)0510
000873824 8564_ $$uhttps://juser.fz-juelich.de/record/873824/files/Wolf%20et%20al%202020%20Front%20Microbiol%2011%2887%29%20with%20SI%20%28003%29.pdf?subformat=pdfa$$xpdfa$$yRestricted$$zStatID:(DE-HGF)0599
000873824 909CO $$ooai:juser.fz-juelich.de:873824$$pdnbdelivery$$pdriver$$pVDB$$popen_access$$popenaire
000873824 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)171102$$aForschungszentrum Jülich$$b0$$kFZJ
000873824 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)128982$$aForschungszentrum Jülich$$b5$$kFZJ
000873824 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)145489$$aForschungszentrum Jülich$$b8$$kFZJ
000873824 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)128943$$aForschungszentrum Jülich$$b9$$kFZJ
000873824 9131_ $$0G:(DE-HGF)POF3-581$$1G:(DE-HGF)POF3-580$$2G:(DE-HGF)POF3-500$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bKey Technologies$$lKey Technologies for the Bioeconomy$$vBiotechnology$$x0
000873824 9141_ $$y2020
000873824 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS
000873824 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews
000873824 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000873824 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bFRONT MICROBIOL : 2017
000873824 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal
000873824 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ
000873824 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000873824 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000873824 915__ $$0StatID:(DE-HGF)9900$$2StatID$$aIF < 5
000873824 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000873824 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bDOAJ : Blind peer review
000873824 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database
000873824 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000873824 915__ $$0StatID:(DE-HGF)0320$$2StatID$$aDBCoverage$$bPubMed Central
000873824 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List
000873824 920__ $$lyes
000873824 9201_ $$0I:(DE-Juel1)IBG-1-20101118$$kIBG-1$$lBiotechnologie$$x0
000873824 980__ $$ajournal
000873824 980__ $$aVDB
000873824 980__ $$aUNRESTRICTED
000873824 980__ $$aI:(DE-Juel1)IBG-1-20101118
000873824 9801_ $$aFullTexts