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@ARTICLE{Battling:873929,
      author       = {Battling, Svenja and Wohlers, Karen and Igwe, Chika and
                      Kranz, Angela and Pesch, Matthias and Wirtz, Astrid and
                      Baumgart, Meike and Büchs, Jochen and Bott, Michael},
      title        = {{N}ovel plasmid-free {G}luconobacter oxydans strains for
                      production of the natural sweetener 5-ketofructose},
      journal      = {Microbial cell factories},
      volume       = {19},
      number       = {1},
      issn         = {1475-2859},
      address      = {London},
      publisher    = {Biomed Central},
      reportid     = {FZJ-2020-01102},
      pages        = {54},
      year         = {2020},
      note         = {Biotechnologie 1},
      abstract     = {5-Ketofructose (5-KF) has recently been identified as a
                      promising non-nutritive natural sweetener. Gluconobacter
                      oxydans strains have been developed that allow efficient
                      production of 5-KF from fructose by plasmid-based expression
                      of the fructose dehydrogenase For plasmid-free 5-KF
                      production, we selected four sites in the genome of G.
                      oxydans IK003.1 and inserted the fdhSCL genes under control
                      of the strong P264 promoter into each of these sites. All
                      four recombinant strains expressed fdhSCL and oxidized
                      fructose to 5-KF, but site-specific differences were
                      observed suggesting that the genomic vicinity influenced
                      gene expression. For further improvement, a second copy of
                      the fdhSCL genes under control of P264 was inserted into the
                      second-best insertion site to obtain strain
                      IK003.1::fdhSCL2. The 5-KF production rate and the 5-KF
                      yield obtained with this double-integration strain were
                      considerably higher than for the single integration strains
                      and approached the values of IK003.1 with plasmid-based
                      fdhSCL expression.We identified four sites in the genome of
                      G. oxydans suitable for expression of heterologous genes and
                      constructed a strain with two genomic copies of the fdhSCL
                      genes enabling efficient plasmid-free 5-KF production. This
                      strain will serve as basis for further metabolic engineering
                      strategies aiming at the use of alternative carbon sources
                      for 5-KF production and for bioprocess optimization.},
      cin          = {IBG-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32131833},
      UT           = {WOS:000521169100001},
      doi          = {10.1186/s12934-020-01310-7},
      url          = {https://juser.fz-juelich.de/record/873929},
}