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000874176 1001_ $$0P:(DE-HGF)0$$aGerkau, Niklas J.$$b0
000874176 245__ $$aRelation between activity‐induced intracellular sodium transients and ATP dynamics in mouse hippocampal neurons
000874176 260__ $$aHoboken, NJ$$bWiley-Blackwell$$c2019
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000874176 520__ $$aExcitatory neuronal activity results in the influx of Na+ through voltage‐ and ligand‐gated channels. Recovery from accompanying increases in intracellular Na+ concentrations ([Na+]i) is mainly mediated by the Na+/K+‐ATPase (NKA) and is one of the major energy‐consuming processes in the brain. Here, we analysed the relation between different patterns of activity‐induced [Na+]i signalling and ATP in mouse hippocampal CA1 pyramidal neurons by Na+ imaging with sodium‐binding benzofurane isophthalate (SBFI) and employing the genetically encoded nanosensor ATeam1.03YEMK (ATeam). In situ calibrations demonstrated a sigmoidal dependence of the ATeam Förster resonance energy transfer ratio on the intracellular ATP concentration ([ATP]i) with an apparent KD of 2.6 mm, indicating its suitability for [ATP]i measurement. Induction of recurrent network activity resulted in global [Na+]i oscillations with amplitudes of ∼10 mm, encompassing somata and dendrites. These were accompanied by a steady decline in [ATP]i by 0.3–0.4 mm in both compartments. Global [Na+]i transients, induced by afferent fibre stimulation or bath application of glutamate, caused delayed, transient decreases in [ATP]i as well. Brief focal glutamate application that evoked transient local Na+ influx into a dendrite, however, did not result in a measurable reduction in [ATP]i. Our results suggest that ATP consumption by the NKA following global [Na+]i transients temporarily overrides its availability, causing a decrease in [ATP]i. Locally restricted Na+ transients, however, do not result in detectable changes in local [ATP]i, suggesting that ATP production, together with rapid intracellular diffusion of both ATP and Na+ from and to unstimulated neighbouring regions, counteracts a local energy shortage under these conditions. 
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000874176 7001_ $$0P:(DE-HGF)0$$aLerchundi, Rodrigo$$b1
000874176 7001_ $$0P:(DE-HGF)0$$aNelson, Joel S. E.$$b2
000874176 7001_ $$00000-0002-9751-5398$$aLantermann, Marina$$b3
000874176 7001_ $$0P:(DE-Juel1)172869$$aMeyer, Jan$$b4
000874176 7001_ $$00000-0002-6327-0089$$aHirrlinger, Johannes$$b5
000874176 7001_ $$0P:(DE-Juel1)IHRS-BioSoft-140013$$aRose, Christine R.$$b6$$eCorresponding author
000874176 773__ $$0PERI:(DE-600)1475290-6$$a10.1113/JP278658$$gVol. 597, no. 23, p. 5687 - 5705$$n23$$p5687 - 5705$$tThe journal of physiology$$v597$$x1469-7793$$y2019
000874176 8564_ $$uhttps://juser.fz-juelich.de/record/874176/files/Gerkau_et_al-2019-The_Journal_of_Physiology.pdf$$yOpenAccess
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