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| 001 | 874176 | ||
| 005 | 20210130004624.0 | ||
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| 100 | 1 | _ | |a Gerkau, Niklas J. |0 P:(DE-HGF)0 |b 0 |
| 245 | _ | _ | |a Relation between activity‐induced intracellular sodium transients and ATP dynamics in mouse hippocampal neurons |
| 260 | _ | _ | |a Hoboken, NJ |c 2019 |b Wiley-Blackwell |
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| 520 | _ | _ | |a Excitatory neuronal activity results in the influx of Na+ through voltage‐ and ligand‐gated channels. Recovery from accompanying increases in intracellular Na+ concentrations ([Na+]i) is mainly mediated by the Na+/K+‐ATPase (NKA) and is one of the major energy‐consuming processes in the brain. Here, we analysed the relation between different patterns of activity‐induced [Na+]i signalling and ATP in mouse hippocampal CA1 pyramidal neurons by Na+ imaging with sodium‐binding benzofurane isophthalate (SBFI) and employing the genetically encoded nanosensor ATeam1.03YEMK (ATeam). In situ calibrations demonstrated a sigmoidal dependence of the ATeam Förster resonance energy transfer ratio on the intracellular ATP concentration ([ATP]i) with an apparent KD of 2.6 mm, indicating its suitability for [ATP]i measurement. Induction of recurrent network activity resulted in global [Na+]i oscillations with amplitudes of ∼10 mm, encompassing somata and dendrites. These were accompanied by a steady decline in [ATP]i by 0.3–0.4 mm in both compartments. Global [Na+]i transients, induced by afferent fibre stimulation or bath application of glutamate, caused delayed, transient decreases in [ATP]i as well. Brief focal glutamate application that evoked transient local Na+ influx into a dendrite, however, did not result in a measurable reduction in [ATP]i. Our results suggest that ATP consumption by the NKA following global [Na+]i transients temporarily overrides its availability, causing a decrease in [ATP]i. Locally restricted Na+ transients, however, do not result in detectable changes in local [ATP]i, suggesting that ATP production, together with rapid intracellular diffusion of both ATP and Na+ from and to unstimulated neighbouring regions, counteracts a local energy shortage under these conditions. |
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| 700 | 1 | _ | |a Meyer, Jan |0 P:(DE-Juel1)172869 |b 4 |
| 700 | 1 | _ | |a Hirrlinger, Johannes |0 0000-0002-6327-0089 |b 5 |
| 700 | 1 | _ | |a Rose, Christine R. |0 P:(DE-Juel1)IHRS-BioSoft-140013 |b 6 |e Corresponding author |
| 773 | _ | _ | |a 10.1113/JP278658 |g Vol. 597, no. 23, p. 5687 - 5705 |0 PERI:(DE-600)1475290-6 |n 23 |p 5687 - 5705 |t The journal of physiology |v 597 |y 2019 |x 1469-7793 |
| 856 | 4 | _ | |y OpenAccess |u https://juser.fz-juelich.de/record/874176/files/Gerkau_et_al-2019-The_Journal_of_Physiology.pdf |
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