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@ARTICLE{Rosenbach:874247,
      author       = {Rosenbach, Hannah and Victor, Julian and Borggräfe, Jan
                      and Biehl, Ralf and Steger, Gerhard and Etzkorn, Manuel and
                      Span, Ingrid},
      title        = {{E}xpanding crystallization tools for nucleic acid
                      complexes using {U}1{A} protein variants},
      journal      = {Journal of structural biology},
      volume       = {210},
      number       = {2},
      issn         = {1047-8477},
      address      = {San Diego, Calif.},
      publisher    = {Elsevier},
      reportid     = {FZJ-2020-01338},
      pages        = {107480 -},
      year         = {2020},
      abstract     = {The major bottlenecks in structure elucidation of nucleic
                      acids are crystallization and phasing. Co-crystallization
                      with proteins is a straight forward approach to overcome
                      these challenges. The human RNA-binding protein U1A has
                      previously been established as crystallization module,
                      however, the absence of UV-active residues and the
                      predetermined architecture in the asymmetric unit constitute
                      clear limitations of the U1A system. Here, we report three
                      crystal structures of tryptophan-containing U1A variants,
                      which expand the crystallization toolbox for nucleic acids.
                      Analysis of the structures complemented by SAXS, NMR
                      spectroscopy, and optical spectroscopy allow for insights
                      into the potential of the U1A variants to serve as
                      crystallization modules for nucleic acids. In addition, we
                      report a fast and efficient protocol for crystallization of
                      RNA by soaking and present a fluorescence-based approach for
                      detecting RNA-binding in crystallo. Our results provide a
                      new tool set for the crystallization of RNA and RNA:DNA
                      complexes.},
      cin          = {ICS-6 / JCNS-1},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ICS-6-20110106 / I:(DE-Juel1)JCNS-1-20110106},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32070773},
      UT           = {WOS:000527725100009},
      doi          = {10.1016/j.jsb.2020.107480},
      url          = {https://juser.fz-juelich.de/record/874247},
}