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@ARTICLE{Li:874551,
      author       = {Li, Wing-Jin and Narancic, Tanja and Kenny, Shane T. and
                      Niehoff, Paul-Joachim and O’Connor, Kevin and Blank, Lars
                      M. and Wierckx, Nick},
      title        = {{U}nraveling 1,4-{B}utanediol {M}etabolism in {P}seudomonas
                      putida {KT}2440},
      journal      = {Frontiers in microbiology},
      volume       = {11},
      issn         = {1664-302X},
      address      = {Lausanne},
      publisher    = {Frontiers Media},
      reportid     = {FZJ-2020-01505},
      pages        = {382},
      year         = {2020},
      note         = {Biotechnologie 1},
      abstract     = {Plastics, in all forms, are a ubiquitous cornerstone of
                      modern civilization. Although humanity undoubtedly benefits
                      from the versatility and durability of plastics, they also
                      cause a tremendous burden for the environment. Bio-upcycling
                      is a promising approach to reduce this burden, especially
                      for polymers that are currently not amenable to mechanical
                      recycling. Wildtype P. putida KT2440 is able to grow on
                      1,4-butanediol as sole carbon source, but only very slowly.
                      Adaptive laboratory evolution (ALE) led to the isolation of
                      several strains with significantly enhanced growth rate and
                      yield. Genome re-sequencing and proteomic analysis were
                      applied to characterize the genomic and metabolic basis of
                      efficient 1,4-butanediol metabolism. Initially,
                      1,4-butanediol is oxidized to 4-hydroxybutyrate, in which
                      the highly expressed dehydrogenase enzymes encoded within
                      the $PP_2674-2680$ ped gene cluster play an essential role.
                      The resulting 4-hydroxybutyrate can be metabolized through
                      three possible pathways: (i) oxidation to succinate, (ii)
                      CoA activation and subsequent oxidation to succinyl-CoA, and
                      (iii) beta oxidation to glycolyl-CoA and acetyl-CoA. The
                      evolved strains were both mutated in a transcriptional
                      regulator $(PP_2046)$ of an operon encoding both
                      beta-oxidation related genes and an alcohol dehydrogenase.
                      When either the regulator or the alcohol dehydrogenase is
                      deleted, no 1,4-butanediol uptake or growth could be
                      detected. Using a reverse engineering approach, $PP_2046$
                      was replaced by a synthetic promotor (14g) to overexpress
                      the downstream operon $(PP_2047-2051),$ thereby enhancing
                      growth on 1,4-butanediol. This work provides a deeper
                      understanding of microbial 1,4-butanediol metabolism in P.
                      putida, which is also expandable to other aliphatic
                      alpha-omega diols. It enables the more efficient metabolism
                      of these diols, thereby enabling biotechnological
                      valorization of plastic monomers in a bio-upcycling
                      approach.},
      cin          = {IBG-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32256468},
      UT           = {WOS:000525716600001},
      doi          = {10.3389/fmicb.2020.00382},
      url          = {https://juser.fz-juelich.de/record/874551},
}