TY  - JOUR
AU  - Pora, Anne
AU  - Yoon, Sungjun
AU  - Dreissen, Georg
AU  - Hoffmann, Bernd
AU  - Merkel, Rudolf
AU  - Windoffer, Reinhard
AU  - Leube, Rudolf E.
TI  - Regulation of keratin network dynamics by the mechanical properties of the environment in migrating cells
JO  - Scientific reports
VL  - 10
IS  - 1
SN  - 2045-2322
CY  - [London]
PB  - Macmillan Publishers Limited, part of Springer Nature
M1  - FZJ-2020-01566
SP  - 4574
PY  - 2020
AB  - Keratin intermediate filaments provide mechanical resilience for epithelia. They are nevertheless highly dynamic and turn over continuously, even in sessile keratinocytes. The aim of this study was to characterize and understand how the dynamic behavior of the keratin cytoskeleton is integrated in migrating cells. By imaging human primary keratinocytes producing fluorescent reporters and by using standardized image analysis we detect inward-directed keratin flow with highest rates in the cell periphery. The keratin flow correlates with speed and trajectory of migration. Changes in fibronectin-coating density and substrate stiffness induces concordant changes in migration speed and keratin flow. When keratinocytes are pseudo-confined on stripes, migration speed and keratin flow are reduced affecting the latter disproportionately. The regulation of keratin flow is linked to the regulation of actin flow. Local speed and direction of keratin and actin flow are very similar in migrating keratinocytes with keratin flow lagging behind actin flow. Conversely, reduced actin flow in areas of high keratin density indicates an inhibitory function of keratins on actin dynamics. Together, we propose that keratins enhance persistence of migration by directing actin dynamics and that the interplay of keratin and actin dynamics is modulated by matrix adhesions.
LB  - PUB:(DE-HGF)16
C6  - pmid:32165652
UR  - <Go to ISI:>//WOS:000520964500026
DO  - DOI:10.1038/s41598-020-61242-5
UR  - https://juser.fz-juelich.de/record/874647
ER  -