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@ARTICLE{Pora:874647,
      author       = {Pora, Anne and Yoon, Sungjun and Dreissen, Georg and
                      Hoffmann, Bernd and Merkel, Rudolf and Windoffer, Reinhard
                      and Leube, Rudolf E.},
      title        = {{R}egulation of keratin network dynamics by the mechanical
                      properties of the environment in migrating cells},
      journal      = {Scientific reports},
      volume       = {10},
      number       = {1},
      issn         = {2045-2322},
      address      = {[London]},
      publisher    = {Macmillan Publishers Limited, part of Springer Nature},
      reportid     = {FZJ-2020-01566},
      pages        = {4574},
      year         = {2020},
      abstract     = {Keratin intermediate filaments provide mechanical
                      resilience for epithelia. They are nevertheless highly
                      dynamic and turn over continuously, even in sessile
                      keratinocytes. The aim of this study was to characterize and
                      understand how the dynamic behavior of the keratin
                      cytoskeleton is integrated in migrating cells. By imaging
                      human primary keratinocytes producing fluorescent reporters
                      and by using standardized image analysis we detect
                      inward-directed keratin flow with highest rates in the cell
                      periphery. The keratin flow correlates with speed and
                      trajectory of migration. Changes in fibronectin-coating
                      density and substrate stiffness induces concordant changes
                      in migration speed and keratin flow. When keratinocytes are
                      pseudo-confined on stripes, migration speed and keratin flow
                      are reduced affecting the latter disproportionately. The
                      regulation of keratin flow is linked to the regulation of
                      actin flow. Local speed and direction of keratin and actin
                      flow are very similar in migrating keratinocytes with
                      keratin flow lagging behind actin flow. Conversely, reduced
                      actin flow in areas of high keratin density indicates an
                      inhibitory function of keratins on actin dynamics. Together,
                      we propose that keratins enhance persistence of migration by
                      directing actin dynamics and that the interplay of keratin
                      and actin dynamics is modulated by matrix adhesions.},
      cin          = {IBI-2},
      ddc          = {600},
      cid          = {I:(DE-Juel1)IBI-2-20200312},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32165652},
      UT           = {WOS:000520964500026},
      doi          = {10.1038/s41598-020-61242-5},
      url          = {https://juser.fz-juelich.de/record/874647},
}