000874661 001__ 874661
000874661 005__ 20220930130234.0
000874661 0247_ $$2doi$$a10.3390/ijms21062244
000874661 0247_ $$2ISSN$$a1422-0067
000874661 0247_ $$2ISSN$$a1661-6596
000874661 0247_ $$2Handle$$a2128/24597
000874661 0247_ $$2pmid$$apmid:32213928
000874661 0247_ $$2WOS$$aWOS:000529890200340
000874661 037__ $$aFZJ-2020-01577
000874661 041__ $$aEnglish
000874661 082__ $$a540
000874661 1001_ $$0P:(DE-Juel1)164475$$aHoffmann, Marco$$b0
000874661 245__ $$aComplex Size and Surface Charge Determine Nucleic Acid Transfer by Fusogenic Liposomes
000874661 260__ $$aBasel$$bMolecular Diversity Preservation International$$c2020
000874661 3367_ $$2DRIVER$$aarticle
000874661 3367_ $$2DataCite$$aOutput Types/Journal article
000874661 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1585149581_14193
000874661 3367_ $$2BibTeX$$aARTICLE
000874661 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000874661 3367_ $$00$$2EndNote$$aJournal Article
000874661 520__ $$aHighly efficient, biocompatible, and fast nucleic acid delivery methods are essential for biomedical applications and research. At present, two main strategies are used to this end. In non-viral transfection liposome- or polymer-based formulations are used to transfer cargo into cells via endocytosis, whereas viral carriers enable direct nucleic acid delivery into the cell cytoplasm. Here, we introduce a new generation of liposomes for nucleic acid delivery, which immediately fuse with the cellular plasma membrane upon contact to transfer the functional nucleic acid directly into the cell cytoplasm. For maximum fusion efficiency combined with high cargo transfer, nucleic acids had to be complexed and partially neutralized before incorporation into fusogenic liposomes. Among the various neutralization agents tested, small, linear, and positively charged polymers yielded the best complex properties. Systematic variation of liposomal composition and nucleic acid complexation identified surface charge as well as particle size as essential parameters for cargo-liposome interaction and subsequent fusion induction. Optimized protocols were tested for the efficient transfer of different kinds of nucleic acids like plasmid DNA, messenger RNA, and short-interfering RNA into various mammalian cells in culture and into primary tissues
000874661 536__ $$0G:(DE-HGF)POF3-552$$a552 - Engineering Cell Function (POF3-552)$$cPOF3-552$$fPOF III$$x0
000874661 588__ $$aDataset connected to CrossRef
000874661 7001_ $$0P:(DE-Juel1)128815$$aHersch, Nils$$b1
000874661 7001_ $$0P:(DE-Juel1)171121$$aGerlach, Sven$$b2
000874661 7001_ $$0P:(DE-Juel1)129308$$aDreissen, Georg$$b3
000874661 7001_ $$0P:(DE-Juel1)144199$$aSpringer, Ronald$$b4
000874661 7001_ $$0P:(DE-Juel1)128833$$aMerkel, Rudolf$$b5
000874661 7001_ $$0P:(DE-Juel1)128805$$aCsiszár, Agnes$$b6
000874661 7001_ $$0P:(DE-Juel1)128817$$aHoffmann, Bernd$$b7$$eCorresponding author
000874661 773__ $$0PERI:(DE-600)2019364-6$$a10.3390/ijms21062244$$gVol. 21, no. 6, p. 2244 -$$n6$$p2244 -$$tInternational journal of molecular sciences$$v21$$x1422-0067$$y2020
000874661 8564_ $$uhttps://juser.fz-juelich.de/record/874661/files/Invoice_ijms-707684.pdf
000874661 8564_ $$uhttps://juser.fz-juelich.de/record/874661/files/ijms-21-02244.pdf$$yOpenAccess
000874661 8564_ $$uhttps://juser.fz-juelich.de/record/874661/files/ijms-21-02244.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000874661 8564_ $$uhttps://juser.fz-juelich.de/record/874661/files/Invoice_ijms-707684.pdf?subformat=pdfa$$xpdfa
000874661 8767_ $$8ijms-707684$$92020-03-21$$d2020-03-24$$eAPC$$jZahlung erfolgt$$pijms-707684
000874661 909CO $$ooai:juser.fz-juelich.de:874661$$popenCost$$pVDB$$pdriver$$pOpenAPC$$popen_access$$popenaire$$pdnbdelivery
000874661 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)164475$$aForschungszentrum Jülich$$b0$$kFZJ
000874661 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)128815$$aForschungszentrum Jülich$$b1$$kFZJ
000874661 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)171121$$aForschungszentrum Jülich$$b2$$kFZJ
000874661 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)129308$$aForschungszentrum Jülich$$b3$$kFZJ
000874661 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)144199$$aForschungszentrum Jülich$$b4$$kFZJ
000874661 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)128833$$aForschungszentrum Jülich$$b5$$kFZJ
000874661 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)128805$$aForschungszentrum Jülich$$b6$$kFZJ
000874661 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)128817$$aForschungszentrum Jülich$$b7$$kFZJ
000874661 9131_ $$0G:(DE-HGF)POF3-552$$1G:(DE-HGF)POF3-550$$2G:(DE-HGF)POF3-500$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bKey Technologies$$lBioSoft – Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences$$vEngineering Cell Function$$x0
000874661 9141_ $$y2020
000874661 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS
000874661 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000874661 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search
000874661 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bINT J MOL SCI : 2017
000874661 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal
000874661 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ
000874661 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000874661 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000874661 915__ $$0StatID:(DE-HGF)9900$$2StatID$$aIF < 5
000874661 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000874661 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC
000874661 915__ $$0StatID:(DE-HGF)1150$$2StatID$$aDBCoverage$$bCurrent Contents - Physical, Chemical and Earth Sciences
000874661 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database
000874661 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000874661 915__ $$0StatID:(DE-HGF)0320$$2StatID$$aDBCoverage$$bPubMed Central
000874661 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List
000874661 920__ $$lyes
000874661 9201_ $$0I:(DE-Juel1)IBI-2-20200312$$kIBI-2$$lMechanobiologie$$x0
000874661 980__ $$ajournal
000874661 980__ $$aVDB
000874661 980__ $$aI:(DE-Juel1)IBI-2-20200312
000874661 980__ $$aAPC
000874661 980__ $$aUNRESTRICTED
000874661 9801_ $$aAPC
000874661 9801_ $$aFullTexts