TY  - JOUR
AU  - Hoffmann, Marco
AU  - Hersch, Nils
AU  - Gerlach, Sven
AU  - Dreissen, Georg
AU  - Springer, Ronald
AU  - Merkel, Rudolf
AU  - Csiszár, Agnes
AU  - Hoffmann, Bernd
TI  - Complex Size and Surface Charge Determine Nucleic Acid Transfer by Fusogenic Liposomes
JO  - International journal of molecular sciences
VL  - 21
IS  - 6
SN  - 1422-0067
CY  - Basel
PB  - Molecular Diversity Preservation International
M1  - FZJ-2020-01577
SP  - 2244 -
PY  - 2020
AB  - Highly efficient, biocompatible, and fast nucleic acid delivery methods are essential for biomedical applications and research. At present, two main strategies are used to this end. In non-viral transfection liposome- or polymer-based formulations are used to transfer cargo into cells via endocytosis, whereas viral carriers enable direct nucleic acid delivery into the cell cytoplasm. Here, we introduce a new generation of liposomes for nucleic acid delivery, which immediately fuse with the cellular plasma membrane upon contact to transfer the functional nucleic acid directly into the cell cytoplasm. For maximum fusion efficiency combined with high cargo transfer, nucleic acids had to be complexed and partially neutralized before incorporation into fusogenic liposomes. Among the various neutralization agents tested, small, linear, and positively charged polymers yielded the best complex properties. Systematic variation of liposomal composition and nucleic acid complexation identified surface charge as well as particle size as essential parameters for cargo-liposome interaction and subsequent fusion induction. Optimized protocols were tested for the efficient transfer of different kinds of nucleic acids like plasmid DNA, messenger RNA, and short-interfering RNA into various mammalian cells in culture and into primary tissues
LB  - PUB:(DE-HGF)16
C6  - pmid:32213928
UR  - <Go to ISI:>//WOS:000529890200340
DO  - DOI:10.3390/ijms21062244
UR  - https://juser.fz-juelich.de/record/874661
ER  -