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@ARTICLE{Hoffmann:874661,
      author       = {Hoffmann, Marco and Hersch, Nils and Gerlach, Sven and
                      Dreissen, Georg and Springer, Ronald and Merkel, Rudolf and
                      Csiszár, Agnes and Hoffmann, Bernd},
      title        = {{C}omplex {S}ize and {S}urface {C}harge {D}etermine
                      {N}ucleic {A}cid {T}ransfer by {F}usogenic {L}iposomes},
      journal      = {International journal of molecular sciences},
      volume       = {21},
      number       = {6},
      issn         = {1422-0067},
      address      = {Basel},
      publisher    = {Molecular Diversity Preservation International},
      reportid     = {FZJ-2020-01577},
      pages        = {2244 -},
      year         = {2020},
      abstract     = {Highly efficient, biocompatible, and fast nucleic acid
                      delivery methods are essential for biomedical applications
                      and research. At present, two main strategies are used to
                      this end. In non-viral transfection liposome- or
                      polymer-based formulations are used to transfer cargo into
                      cells via endocytosis, whereas viral carriers enable direct
                      nucleic acid delivery into the cell cytoplasm. Here, we
                      introduce a new generation of liposomes for nucleic acid
                      delivery, which immediately fuse with the cellular plasma
                      membrane upon contact to transfer the functional nucleic
                      acid directly into the cell cytoplasm. For maximum fusion
                      efficiency combined with high cargo transfer, nucleic acids
                      had to be complexed and partially neutralized before
                      incorporation into fusogenic liposomes. Among the various
                      neutralization agents tested, small, linear, and positively
                      charged polymers yielded the best complex properties.
                      Systematic variation of liposomal composition and nucleic
                      acid complexation identified surface charge as well as
                      particle size as essential parameters for cargo-liposome
                      interaction and subsequent fusion induction. Optimized
                      protocols were tested for the efficient transfer of
                      different kinds of nucleic acids like plasmid DNA, messenger
                      RNA, and short-interfering RNA into various mammalian cells
                      in culture and into primary tissues},
      cin          = {IBI-2},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBI-2-20200312},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32213928},
      UT           = {WOS:000529890200340},
      doi          = {10.3390/ijms21062244},
      url          = {https://juser.fz-juelich.de/record/874661},
}