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@ARTICLE{Habicher:874872,
      author       = {Habicher, Tobias and Rauls, Edward K. A. and Egidi,
                      Franziska and Keil, Timm and Klein, Tobias and Daub, Andreas
                      and Büchs, Jochen},
      title        = {{E}stablishing a {F}ed‐{B}atch {P}rocess for {P}rotease
                      {E}xpression with {B}acillus licheniformis in
                      {P}olymer‐{B}ased {C}ontrolled‐{R}elease {M}icrotiter
                      {P}lates},
      journal      = {Biotechnology journal},
      volume       = {15},
      number       = {2},
      issn         = {1860-7314},
      address      = {Weinheim},
      publisher    = {Wiley-VCH},
      reportid     = {FZJ-2020-01677},
      pages        = {1900088 -},
      year         = {2020},
      abstract     = {Introducing fed‐batch mode in early stages of development
                      projects is crucial for establishing comparable conditions
                      to industrial fed‐batch fermentation processes. Therefore,
                      cost efficient and easy to use small‐scale fed‐batch
                      systems that can be integrated into existing laboratory
                      equipment and workflows are required. Recently, a novel
                      polymer‐based controlled‐release fed‐batch microtiter
                      plate is described. In this work, the polymer‐based
                      controlled‐release fed‐batch microtiter plate is used to
                      investigate fed‐batch cultivations of a protease producing
                      Bacillus licheniformis culture. Therefore, the oxygen
                      transfer rate (OTR) is online‐monitored within each well
                      of the polymer‐based controlled‐release fed‐batch
                      microtiter plate using a µRAMOS device. Cultivations in
                      five individual polymer‐based controlled‐release
                      fed‐batch microtiter plates of two production lots show
                      good reproducibility with a mean coefficient of variation of
                      $9.2\%.$ Decreasing initial biomass concentrations prolongs
                      batch phase while simultaneously postponing the fed‐batch
                      phase. The initial liquid filling volume affects the
                      volumetric release rate, which is directly translated in
                      different OTR levels of the fed‐batch phase. An increasing
                      initial osmotic pressure within the mineral medium decreases
                      both glucose release and protease yield. With the volumetric
                      glucose release rate as scale‐up criterion, microtiter
                      plate‐ and shake flask‐based fed‐batch cultivations
                      are highly comparable. On basis of the small‐scale
                      fed‐batch cultivations, a mechanistic model is established
                      and validated. Model‐based simulations coincide well with
                      the experimentally acquired data.},
      cin          = {IEK-14},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IEK-14-20191129},
      pnm          = {134 - Electrolysis and Hydrogen (POF3-134)},
      pid          = {G:(DE-HGF)POF3-134},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31471944},
      UT           = {WOS:000511444500003},
      doi          = {10.1002/biot.201900088},
      url          = {https://juser.fz-juelich.de/record/874872},
}