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@INPROCEEDINGS{Pflugfelder:874925,
      author       = {Pflugfelder, Daniel and Kochs, Johannes and Koller, Robert
                      and Jahnke, Siegfried and Mohl, Carola and Faßbender, Heike
                      and Schurr, Ulrich and Watt, Michelle and van Dusschoten,
                      Dagmar},
      title        = {{G}enotypic differences in early wheat seedling
                      establishment in natural soil investigated using
                      non-invasive 3{D} magnetic resonance imaging},
      reportid     = {FZJ-2020-01709},
      year         = {2020},
      abstract     = {Seed germination and seedling establishment are the first
                      important steps in a plant's growth cycle. We developed a
                      measurement pipeline to quantify the early stages of root
                      development in young seedlings in natural soil. We used
                      non-invasive magnetic resonance imaging (MRI), providing us
                      with 3D information about root structures. By growing up to
                      18 seedlings per pot (Ø=12.5cm, 10cm height) and by
                      focusing on early growth (up to 4 days after start of
                      germination), a much higher plant throughput compared to
                      traditional 3D root measurement protocols in soil was
                      achieved. Due to the high temporal resolution of the
                      acquired data sets (4 images per day), dynamic traits such
                      as shoot and root emergence time were obtained accurately.
                      We used this 'deep phenotyping' approach, with multiple
                      temporal and spatial layers of data, to investigate
                      genotypic differences using the 8 parent lines of the NIAB
                      MAGIC population. Clear genotypic differences in structural
                      (e.g. root angle, root lengths, and number) and temporal
                      (e.g. time of root emergence, shoot emergence) were
                      quantified. We identified different growth strategies among
                      the parent genotypes by correlating the temporal development
                      of traits. This new MRI method offers a promising tool for
                      high throughput root seedling screening in natural soil
                      environments, along with opportunities to discover new
                      traits based on dynamics of root and shoot development. This
                      work was financially supported by BASF.},
      month         = {Feb},
      date          = {2020-02-24},
      organization  = {Phenome2020, Tucson (USA), 24 Feb 2020
                       - 27 Feb 2020},
      subtyp        = {Invited},
      cin          = {IBG-2},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {582 - Plant Science (POF3-582)},
      pid          = {G:(DE-HGF)POF3-582},
      typ          = {PUB:(DE-HGF)6},
      url          = {https://juser.fz-juelich.de/record/874925},
}