Gast ::
| Hauptseite > Publikationsdatenbank > Molecular mechanism of light-driven sodium pumping > print |
| 001 | 874981 | ||
| 005 | 20220930130235.0 | ||
| 024 | 7 | _ | |a 10.1038/s41467-020-16032-y |2 doi |
| 024 | 7 | _ | |a 2128/25096 |2 Handle |
| 024 | 7 | _ | |a altmetric:80986960 |2 altmetric |
| 024 | 7 | _ | |a pmid:32358514 |2 pmid |
| 024 | 7 | _ | |a WOS:000531425700018 |2 WOS |
| 037 | _ | _ | |a FZJ-2020-01746 |
| 082 | _ | _ | |a 500 |
| 100 | 1 | _ | |a Kovalev, Kirill |0 P:(DE-Juel1)169220 |b 0 |
| 245 | _ | _ | |a Molecular mechanism of light-driven sodium pumping |
| 260 | _ | _ | |a [London] |c 2020 |b Nature Publishing Group UK |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1592485877_23512 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a The light-driven sodium-pumping rhodopsin KR2 from Krokinobacter eikastus is the only non-proton cation active transporter with demonstrated potential for optogenetics. However, the existing structural data on KR2 correspond exclusively to its ground state, and show no sodium inside the protein, which hampers the understanding of sodium-pumping mechanism. Here we present crystal structure of the O-intermediate of the physiologically relevant pentameric form of KR2 at the resolution of 2.1 Å, revealing a sodium ion near the retinal Schiff base, coordinated by N112 and D116 of the characteristic NDQ triad. We also obtained crystal structures of D116N and H30A variants, conducted metadynamics simulations and measured pumping activities of putative pathway mutants to demonstrate that sodium release likely proceeds alongside Q78 towards the structural sodium ion bound between KR2 protomers. Our findings highlight the importance of pentameric assembly for sodium pump function, and may be used for rational engineering of enhanced optogenetic tools. |
| 536 | _ | _ | |a 551 - Functional Macromolecules and Complexes (POF3-551) |0 G:(DE-HGF)POF3-551 |c POF3-551 |f POF III |x 0 |
| 588 | _ | _ | |a Dataset connected to CrossRef |
| 700 | 1 | _ | |a Astashkin, Roman |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Gushchin, Ivan |0 P:(DE-HGF)0 |b 2 |
| 700 | 1 | _ | |a Orekhov, Philipp |0 0000-0003-4078-4762 |b 3 |
| 700 | 1 | _ | |a Volkov, Dmytro |0 P:(DE-Juel1)176195 |b 4 |
| 700 | 1 | _ | |a Zinovev, Egor |0 P:(DE-Juel1)174058 |b 5 |
| 700 | 1 | _ | |a Marin, Egor |0 0000-0003-2369-1732 |b 6 |
| 700 | 1 | _ | |a Rulev, Maksim |0 P:(DE-HGF)0 |b 7 |
| 700 | 1 | _ | |a Alekseev, Alexey |0 P:(DE-Juel1)169221 |b 8 |
| 700 | 1 | _ | |a Royant, Antoine |0 P:(DE-HGF)0 |b 9 |
| 700 | 1 | _ | |a Carpentier, Philippe |0 P:(DE-HGF)0 |b 10 |
| 700 | 1 | _ | |a Vaganova, Svetlana |0 P:(DE-Juel1)176102 |b 11 |
| 700 | 1 | _ | |a Zabelskii, Dmitrii |0 P:(DE-Juel1)176570 |b 12 |
| 700 | 1 | _ | |a Baeken, Christian |0 P:(DE-Juel1)131948 |b 13 |
| 700 | 1 | _ | |a Sergeev, Ilya |0 P:(DE-Juel1)136714 |b 14 |
| 700 | 1 | _ | |a Balandin, Taras |0 P:(DE-Juel1)131949 |b 15 |
| 700 | 1 | _ | |a Bourenkov, Gleb |0 P:(DE-HGF)0 |b 16 |
| 700 | 1 | _ | |a Carpena, Xavier |0 0000-0002-8646-0440 |b 17 |
| 700 | 1 | _ | |a Boer, Roeland |0 0000-0001-5949-6627 |b 18 |
| 700 | 1 | _ | |a Maliar, Nina |0 0000-0002-9216-4525 |b 19 |
| 700 | 1 | _ | |a Borshchevskiy, Valentin |0 P:(DE-Juel1)179072 |b 20 |
| 700 | 1 | _ | |a Büldt, Georg |0 P:(DE-Juel1)131957 |b 21 |
| 700 | 1 | _ | |a Bamberg, Ernst |0 P:(DE-HGF)0 |b 22 |
| 700 | 1 | _ | |a Gordeliy, Valentin |0 P:(DE-Juel1)131964 |b 23 |e Corresponding author |
| 773 | _ | _ | |a 10.1038/s41467-020-16032-y |g Vol. 11, no. 1, p. 2137 |0 PERI:(DE-600)2553671-0 |n 1 |p 2137 |t Nature Communications |v 11 |y 2020 |x 2041-1723 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/874981/files/Invoice_2676181679.pdf |
| 856 | 4 | _ | |x pdfa |u https://juser.fz-juelich.de/record/874981/files/Invoice_2676181679.pdf?subformat=pdfa |
| 856 | 4 | _ | |y OpenAccess |u https://juser.fz-juelich.de/record/874981/files/s41467-020-16032-y.pdf |
| 856 | 4 | _ | |y OpenAccess |x pdfa |u https://juser.fz-juelich.de/record/874981/files/s41467-020-16032-y.pdf?subformat=pdfa |
| 909 | C | O | |o oai:juser.fz-juelich.de:874981 |p openaire |p open_access |p OpenAPC |p driver |p VDB |p openCost |p dnbdelivery |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 0 |6 P:(DE-Juel1)169220 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 4 |6 P:(DE-Juel1)176195 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 5 |6 P:(DE-Juel1)174058 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 11 |6 P:(DE-Juel1)176102 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 12 |6 P:(DE-Juel1)176570 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 13 |6 P:(DE-Juel1)131948 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 15 |6 P:(DE-Juel1)131949 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 20 |6 P:(DE-Juel1)179072 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 23 |6 P:(DE-Juel1)131964 |
| 913 | 1 | _ | |a DE-HGF |b Key Technologies |l BioSoft – Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences |1 G:(DE-HGF)POF3-550 |0 G:(DE-HGF)POF3-551 |2 G:(DE-HGF)POF3-500 |v Functional Macromolecules and Complexes |x 0 |4 G:(DE-HGF)POF |3 G:(DE-HGF)POF3 |
| 914 | 1 | _ | |y 2020 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0200 |2 StatID |b SCOPUS |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1030 |2 StatID |b Current Contents - Life Sciences |
| 915 | _ | _ | |a Creative Commons Attribution CC BY 4.0 |0 LIC:(DE-HGF)CCBY4 |2 HGFVOC |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1040 |2 StatID |b Zoological Record |
| 915 | _ | _ | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |b NAT COMMUN : 2017 |
| 915 | _ | _ | |a IF >= 10 |0 StatID:(DE-HGF)9910 |2 StatID |b NAT COMMUN : 2017 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0501 |2 StatID |b DOAJ Seal |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0500 |2 StatID |b DOAJ |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0110 |2 StatID |b Science Citation Index |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0111 |2 StatID |b Science Citation Index Expanded |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1150 |2 StatID |b Current Contents - Physical, Chemical and Earth Sciences |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0150 |2 StatID |b Web of Science Core Collection |
| 915 | _ | _ | |a OpenAccess |0 StatID:(DE-HGF)0510 |2 StatID |
| 915 | _ | _ | |a Peer Review |0 StatID:(DE-HGF)0030 |2 StatID |b DOAJ : Blind peer review |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1060 |2 StatID |b Current Contents - Agriculture, Biology and Environmental Sciences |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0310 |2 StatID |b NCBI Molecular Biology Database |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1050 |2 StatID |b BIOSIS Previews |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0320 |2 StatID |b PubMed Central |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0199 |2 StatID |b Clarivate Analytics Master Journal List |
| 920 | 1 | _ | |0 I:(DE-Juel1)IBI-7-20200312 |k IBI-7 |l Strukturbiochemie |x 0 |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a UNRESTRICTED |
| 980 | _ | _ | |a I:(DE-Juel1)IBI-7-20200312 |
| 980 | _ | _ | |a APC |
| 980 | 1 | _ | |a APC |
| 980 | 1 | _ | |a FullTexts |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|