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@ARTICLE{Kberl:875079,
      author       = {Küberl, Andreas and Mengus-Kaya, Aliye and Polen, Tino and
                      Bott, Michael},
      title        = {{T}he iron deficiency response of {C}orynebacterium
                      glutamicum and a link to thiamine biosynthesis},
      journal      = {Applied and environmental microbiology},
      volume       = {86},
      issn         = {1098-5336},
      address      = {Washington, DC [u.a.]},
      publisher    = {Soc.},
      reportid     = {FZJ-2020-01788},
      pages        = {e00065-20},
      year         = {2020},
      note         = {Biotechnologie 1},
      abstract     = {The response to iron limitation of the Gram-positive soil
                      bacterium Corynebacterium glutamicum was analyzed with
                      respect to secreted metabolites, the transcriptome, and the
                      proteome. During growth in glucose minimal medium, iron
                      limitation caused a shift from lactate to pyruvate as the
                      major secreted organic acid complemented by l-alanine and
                      2-oxoglutarate. Transcriptome and proteome analyses revealed
                      that a pronounced iron starvation response governed by the
                      transcriptional regulators DtxR and RipA was detectable in
                      the late, but not in the early, exponential-growth phase. A
                      link between iron starvation and thiamine pyrophosphate
                      (TPP) biosynthesis was uncovered by the strong upregulation
                      of thiC. As phosphomethylpyrimidine synthase (ThiC) contains
                      an iron-sulfur cluster, limiting activities of the
                      TPP-dependent pyruvate–2-oxoglutarate dehydrogenase
                      supercomplex probably cause the excretion of pyruvate and
                      2-oxoglutarate. In line with this explanation, thiamine
                      supplementation could strongly diminish the secretion of
                      these acids. The upregulation of thiC and other genes
                      involved in thiamine biosynthesis and transport is
                      presumably due to TPP riboswitches present at the 5′ end
                      of the corresponding operons. The results obtained in this
                      study provide new insights into iron homeostasis in C.
                      glutamicum and demonstrate that the metabolic consequences
                      of iron limitation can be due to the iron dependency of
                      coenzyme biosynthesis.},
      cin          = {IBG-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {32144105},
      UT           = {WOS:000534070600001},
      doi          = {10.1128/AEM.00065-20},
      url          = {https://juser.fz-juelich.de/record/875079},
}