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001     875079
005     20210518171142.0
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100 1 _ |a Küberl, Andreas
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245 _ _ |a The iron deficiency response of Corynebacterium glutamicum and a link to thiamine biosynthesis
260 _ _ |a Washington, DC [u.a.]
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500 _ _ |a Biotechnologie 1
520 _ _ |a The response to iron limitation of the Gram-positive soil bacterium Corynebacterium glutamicum was analyzed with respect to secreted metabolites, the transcriptome, and the proteome. During growth in glucose minimal medium, iron limitation caused a shift from lactate to pyruvate as the major secreted organic acid complemented by l-alanine and 2-oxoglutarate. Transcriptome and proteome analyses revealed that a pronounced iron starvation response governed by the transcriptional regulators DtxR and RipA was detectable in the late, but not in the early, exponential-growth phase. A link between iron starvation and thiamine pyrophosphate (TPP) biosynthesis was uncovered by the strong upregulation of thiC. As phosphomethylpyrimidine synthase (ThiC) contains an iron-sulfur cluster, limiting activities of the TPP-dependent pyruvate–2-oxoglutarate dehydrogenase supercomplex probably cause the excretion of pyruvate and 2-oxoglutarate. In line with this explanation, thiamine supplementation could strongly diminish the secretion of these acids. The upregulation of thiC and other genes involved in thiamine biosynthesis and transport is presumably due to TPP riboswitches present at the 5′ end of the corresponding operons. The results obtained in this study provide new insights into iron homeostasis in C. glutamicum and demonstrate that the metabolic consequences of iron limitation can be due to the iron dependency of coenzyme biosynthesis.
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700 1 _ |a Mengus-Kaya, Aliye
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700 1 _ |a Polen, Tino
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700 1 _ |a Bott, Michael
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856 4 _ |y Published on 2020-05-05. Available in OpenAccess from 2020-11-05.
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