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| 001 | 875083 | ||
| 005 | 20210130004842.0 | ||
| 024 | 7 | _ | |a 10.1002/smll.201906670 |2 doi |
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| 037 | _ | _ | |a FZJ-2020-01792 |
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| 100 | 1 | _ | |a Täuber, Sarah |0 P:(DE-HGF)0 |b 0 |
| 245 | _ | _ | |a Dynamic environmental control in microfluidic single‐cell cultivations: From concepts to applications |
| 260 | _ | _ | |a Weinheim |c 2020 |b Wiley-VCH |
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| 520 | _ | _ | |a Microfluidic single‐cell cultivation (MSCC) is an emerging field within fundamental as well as applied biology. During the last years, most MSCCs were performed at constant environmental conditions. Recently, MSCC at oscillating and dynamic environmental conditions has started to gain significant interest in the research community for the investigation of cellular behavior. Herein, an overview of this topic is given and microfluidic concepts that enable oscillating and dynamic control of environmental conditions with a focus on medium conditions are discussed, and their application in single‐cell research for the cultivation of both mammalian and microbial cell systems is demonstrated. Furthermore, perspectives for performing MSCC at complex dynamic environmental profiles of single parameters and multiparameters (e.g., pH and O2) in amplitude and time are discussed. The technical progress in this field provides completely new experimental approaches and lays the foundation for systematic analysis of cellular metabolism at fluctuating environments. |
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| 700 | 1 | _ | |a von Lieres, Eric |0 P:(DE-Juel1)129081 |b 1 |u fzj |
| 700 | 1 | _ | |a Grünberger, Alexander |0 P:(DE-Juel1)143612 |b 2 |e Corresponding author |
| 773 | _ | _ | |a 10.1002/smll.201906670 |g p. 1906670 - |0 PERI:(DE-600)2168935-0 |n 16 |p 1906670 |t Small |v 16 |y 2020 |x 1613-6829 |
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