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@ARTICLE{He:875084,
author = {He, Qiao-Le and von Lieres, Eric and Sun, Zhaoxi and Zhao,
Liming},
title = {{M}odel-based process design of a ternary protein
separation using multi-step gradient ion-exchange {SMB}
chromatography},
journal = {Computers $\&$ chemical engineering},
volume = {138},
issn = {0098-1354},
address = {Amsterdam [u.a.]},
publisher = {Elsevier Science},
reportid = {FZJ-2020-01793},
pages = {106851},
year = {2020},
abstract = {Model-based process design of ion-exchange simulated moving
bed (iex-smb) chromatography for center-cut separation of
proteins is studied. Use of nonlinear binding models that
describe more accurate adsorption behaviors of
macro-molecules could make it impossible to utilize triangle
theory to obtain operating parameters. Moreover, triangle
theory provides no rules to design salt profiles in iex-smb.
In the modeling study here, proteins (i.e., ribonuclease,
cytochrome and lysozyme) on chromatographic columns packed
with strong cation-exchanger SP Sepharose FF is used as an
example system. The general rate model with steric
mass-action kinetics was used; two closed-loop iex-smb
network schemes were investigated (i.e., cascade and
eight-zone schemes). Performance of the iex-smb schemes was
examined with respect to multi-objective indicators (i.e.,
purity and yield) and productivity, and compared to a single
column batch system with the same amount of resin utilized.
A multi-objective sampling algorithm, Markov Chain Monte
Carlo (mcmc), was used to generate samples for constructing
Pareto optimal fronts. mcmc serves on the sampling purpose,
which is interested in sampling the Pareto optimal points as
well as those near Pareto optimal. Pareto fronts of the
three schemes provide full information on the trade-off
between the conflicting indicators, purity and yield here.
The results indicate that the cascade iex-smb scheme and the
integrated eight-zone iex-smb scheme have similar
performance and that both outperforms the single column
batch system.},
cin = {IBG-1},
ddc = {660},
cid = {I:(DE-Juel1)IBG-1-20101118},
pnm = {583 - Innovative Synergisms (POF3-583)},
pid = {G:(DE-HGF)POF3-583},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000538049100009},
doi = {10.1016/j.compchemeng.2020.106851},
url = {https://juser.fz-juelich.de/record/875084},
}