TY  - JOUR
AU  - Baumer, Benedikt
AU  - Classen, Thomas
AU  - Pohl, Martina
AU  - Pietruszka, Jörg
TI  - Efficient Nicotinamide Adenine Dinucleotide Phosphate [NADP(H)] Recycling in Closed‐Loop Continuous Flow Biocatalysis
JO  - Advanced synthesis & catalysis
VL  - 362
IS  - 14
SN  - 1615-4169
CY  - Weinheim
PB  - Wiley-VCH
M1  - FZJ-2020-01828
SP  - 2894-2901
PY  - 2020
AB  - Biocatalytic redox reactions regularly depend on expensive cofactors that require recycling. For continuous conversions in flow chemistry, this is often an obstacle since the cofactor is washed away. Here, we present a quasi‐stationary recycling system for nicotinamide adenine dinucleotide phosphate utilizing an immobilized alcohol dehydrogenase. Four model substrates were reduced with high enantioselectivity as a proof of concept. The two‐phase system enables continuous production as well as quick substrate changes. This setup may serve as a general cofactor regeneration module for continuous biocatalytic devices employing (co‐)substrates being miscible in organic solvent. The system resulted in space‐time yields up to 117 g L−1 h−1 and total turnover numbers for nicotinamide adenine dinucleotide phosphate higher than 12,000 mol/mol are possible.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000555591000012
DO  - DOI:10.1002/adsc.202000058
UR  - https://juser.fz-juelich.de/record/875135
ER  -