%0 Journal Article
%A Muschallik, Lukas
%A Molinnus, Denise
%A Jablonski, Melanie
%A Kipp, Carina Ronja
%A Bongaerts, Johannes
%A Pohl, Martina
%A Wagner, Torsten
%A Schöning, Michael J.
%A Selmer, Thorsten
%A Siegert, Petra
%T Synthesis of α-hydroxy ketones and vicinal ( R , R )-diols by Bacillus clausii DSM 8716 T butanediol dehydrogenase
%J RSC Advances
%V 10
%N 21
%@ 2046-2069
%C London
%I RSC Publishing
%M FZJ-2020-01831
%P 12206 - 12216
%D 2020
%X α-hydroxy ketones (HK) and 1,2-diols are important building blocks for fine chemical synthesis. Here, we describe the R-selective 2,3-butanediol dehydrogenase from B. clausii DSM 8716T (BcBDH) that belongs to the metal-dependent medium chain dehydrogenases/reductases family (MDR) and catalyzes the selective asymmetric reduction of prochiral 1,2-diketones to the corresponding HK and, in some cases, the reduction of the same to the corresponding 1,2-diols. Aliphatic diketones, like 2,3-pentanedione, 2,3-hexanedione, 5-methyl-2,3-hexanedione, 3,4-hexanedione and 2,3-heptanedione are well transformed. In addition, surprisingly alkyl phenyl dicarbonyls, like 2-hydroxy-1-phenylpropan-1-one and phenylglyoxal are accepted, whereas their derivatives with two phenyl groups are not substrates. Supplementation of Mn2+ (1 mM) increases BcBDH's activity in biotransformations. Furthermore, the biocatalytic reduction of 5-methyl-2,3-hexanedione to mainly 5-methyl-3-hydroxy-2-hexanone with only small amounts of 5-methyl-2-hydroxy-3-hexanone within an enzyme membrane reactor is demonstrated.
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000526839700010
%R 10.1039/D0RA02066D
%U https://juser.fz-juelich.de/record/875139